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Simplifying Muscle Cell Generation

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AMSBIO offers a Skeletal Muscle Differentiation kit that is designed to enable the differentiation of human pluripotent stem cells to skeletal muscle myotubes with high yields, without cell sorting or genetic manipulation. Myotubes are contractile, express typical muscle markers that show striated sarcomeres.

Until recently methods of studying muscular disease and potential therapies were dependent on invasive muscle biopsies to produce limited batches of primary cells. Use of primary cells presents challenges, not only in the collection process but also related to inconsistencies in cell growth, behavior and life span, making it difficult to generate reliable experimental models.

Using AMSBIO's revolutionary Skeletal Muscle Differentiation kit allows researchers to generate muscle from human pluripotent stem cells in 3 easy steps, via satellite-like or progenitor cells and myoblasts that then fuse to multinucleated myotubes in the third step.

Tested on a wide range of human embryonic and induced pluripotent stem cell lines the new kit follows a simple 3-step process of media changes and cell passaging. Eliminating the need for cell sorting or transfection of myogenic transcription factors, the Skeletal Muscle Differentiation kit protocol aims to generate a highly pure population of approximately 70 percent skeletal muscle myotubes in a reproducible fashion.