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Lenti-X™ Transduction Sponge
The Lenti-X™ Transduction Sponge is a novel technology that allows you to achieve microfluidics-driven lentiviral transduction efficiency gains without the use of microfluidics-based instrumentation. With one sponge, you can transduce 105–107 cells without the need for time-consuming spinoculation or harsh chemical transduction enhancers that require optimization to retain cell viability. The Lenti-X Transduction Sponge enables you to effectively transduce a wide variety of cell types, including cell lines and difficult-to-transduce primary cells.
How does the Lenti-X Transduction Sponge work?
A mixture of target cells and lentivirus is added to the sponge and incubated for 1 hr at 37°C allowing the mixture to be absorbed. Following absorption, cell culture media is added to the sponge and then incubated at 37°C for 16–24 hr.
The following day, easily release the transduced cells by dissolving the sponge in a special release buffer. The cells can then be replated for further expansion and downstream applications. The user-friendly protocol minimizes cell handling and requires smaller total reaction volumes, reducing the amount of virus needed. The Lenti-X Transduction Sponge consistently delivers transduction efficiencies comparable to or superior to traditional methods.
