MSC NutriStem®, a complete xeno-free, serum-free system for the growth and expansion of hMSCs content piece image

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MSC NutriStem®, a complete xeno-free, serum-free system for the growth and expansion of hMSCs

Name: MSC NutriStem®, a complete xeno-free, serum-free system for the growth and expansion of hMSCs
Brand: Sartorius

MSC NutriStem® XF Medium

REDEFINING STEM CELL EXCELLENCE AND ADVANCING CLINICAL APPLICATIONS

A defined, serum-free and xeno-free culture system optimized for the growth and expansion of hMSCs from a variety of sources: placenta (hMSC-PL), adipose-tissue (hMSC-AT), Wharton jelly (hMSC-WJ) and bone marrow (hMSC-BM). NutriStem® MSC culture system is developed specifically for the attachment, dissociation and freezing, as well as enabling long-term growth of hMSCs while retaining self-renewal and multi-lineage differentiation potential.

Defined, serum-free, xeno-free reagents are essential tools for all human mesenchymal stem cell (hMSC) research having potential clinical applications. The NutriStem® MSC Culture System includes defined reagents ideal for translational research use.

hMSCs cultured in serum-free, xeno-free MSC NutriStem® XF Medium show superior proliferation and self-renewal potential in comparison to serum-containing media and other serum-free media. In addition, hMSCs maintain their proper fibroblast-like cell morphology, tri-lineage differentiation potential, and demonstrate normal hMSC marker profiles and karyotypic stability over long-term culture.

Features

• Serum-Free (SF), Xeno-Free (XF), defined formulation

• Drug Master File (DMF) available

• hMSCs demonstrate superior proliferation

• Supports long-term growth and self-renewal of hMSCs from multiple sources

• Proper hMSC characteristics and multilineage differentiation potential are maintained over long-term culture

• hMSCs express high percentage of MSC surface markers and do not express hematopoeitic markers

• Complete media kit; no additional supplements are required

Cells cultured in serum-containing medium do not require an adaptation phase when transitioning to serum-free MSC NutriStem® XF Medium.

Initial Isolation:

hMSC from various sources (hMSC-PL, hMSC-AT, hMSC-WJ, hMSC-BM) can be efficiently isolated using MSC NutriStem® XF on pre-coated dishes. Addition of 2-2.5% human AB serum may be required for certain tissues. Using MSC NutriStem® XF for isolation of hMSC enhances purity of MSC populations in earlier passages and increases the number of hMSC in comparison to FBS-containing medium.

Expansion:

hMSC cultured in MSC NutriStem® XF exhibit a high proliferation rate and long term growth in comparison to competitors’ media (see figure 2).

MSC NutriStem® XF is optimized for hMSC from various sources

MSC NutriStem® XF promotes proliferation of hMSC from a variety of sources while maintaining normal fibroblast-like, spindle shape cell morphology (see figure 3).

hMSC Differentiation potential

hMSC cultured in MSC NutriStem® XF maintain their trilineage differentiation potential (see figure 4).

References:

1. S. Bobis-Wozowicz et al. Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles. Journal of Molecular Medicine, 2016

2. K.Y. Tan et al. Serum-free media formulations are cell line–specific and require optimization for microcarrier culture. Cytotherapy, 2015

3. Cai, Zhen, et al. Chondrogenesis of Human Adipose-Derived Stem Cells by In Vivo Co-graft with Auricular Chondrocytes from Microtia. Aesthetic plastic surgery 39.3 (2015): 431-439.

4. A. Jarmalavičiūtė et al., Exosomes from dental pulp stem cells rescue human dopaminergic neurons from 6-hydroxy-dopamine–induced apoptosis. Cytotherapy, 2015

5. M.Pokrywczynska et al., Transdifferentiation of Bone Marrow Mesenchymal Stem Cells into the Islet-Like Cells: the Role of Extracellular Matrix Proteins. Archivum Immunologiae et Therapiae Experimentalis, May 2015

6. U Pivoraitė et. al., Exosomes from Human Dental Pulp Stem Cells Suppress Carrageenan-Induced Acute Inflammation in Mice. Inflammation, April 2015

7. S.H. Mei, et al. Isolation and large-scale expansion of bone marrow-derived mesenchymal stem cells with serum-free media under GMP-compliance. Cytotherapy, Volume 16, Issue 4, Supplement , Page S111, April 2014

8. Li-Yi Sun, et al.Expansion of Semi-Automatic Processed Human Adipose-Derived Stem Cells in Medium Supplemented with Autologous Serum and Antioxidants. Stem Cell Research & Therapy, 2014, 4:4

9. Allen Kuan-Liang Chen, et al.Application of human mesenchymal and pluripotent stem cell microcarrier cultures in cellular therapy: Achievements and future direction. Biotechnology Advances, Volume 31, Issue 7, 15 November 2013, Pages 1032–1046

For more references please refer to http://www.bioind.com/israel/products/stem-cell-research/stem-cell-culture/human-mesenchymal-stem-cells/nutristem-msc-medium/

About Sartorius

We are living in the bio-century. Global medical knowledge is expanding at a rapid pace. But despite huge breakthroughs, many diseases, such as cancer, rheumatism or Alzheimer's, are still not curable. Sartorius is part of the solution. We help to ensure that new scientific discoveries can be translated more quickly into effective patient care. With technologies, platforms and partnerships for a common goal: better health for more people.

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