When developing diagnostic tests or evaluating results, it is important to understand how reliable those tests and therefore the results you are obtaining are. By using samples of known disease status, values such as sensitivity and specificity can be calculated that allow you to evaluate just that.
What do sensitivity values tell you?
The sensitivity of a test is also called the true positive rate (TPR) and is the proportion of samples that are genuinely positive that give a positive result using the test in question. For example, a test that correctly identifies all positive samples in a panel is very sensitive. Another test that only detects 60 % of the positive samples in the panel would be deemed to have lower sensitivity as it is missing positives and giving higher a false negative rate (FNR). Also referred to as type II errors, false negatives are the failure to reject a false null hypothesis (the null hypothesis being that the sample is negative).
What do specificity measures tell you?
The specificity of a test, also referred to as the true negative rate (TNR), is the proportion of samples that test negative using the test in question that are genuinely negative. For example, a test that identifies all healthy people as being negative for a particular illness is very specific. Another test that incorrectly identifies 30 % of healthy people as having the condition would be deemed to be less specific, having a higher false positive rate (FPR). Also referred to as type I errors, false positives are the rejection of a true null hypothesis (the null hypothesis being that the sample is negative).
How do I calculate sensitivity and specificity values?
An ideal test rarely overlooks the thing you are looking for (i.e., it is sensitive) and rarely mistakes it for something else (i.e. it is specific). Therefore, when evaluating diagnostic tests, it is important to calculate the sensitivity and specificity for that test to determine its effectiveness.
The sensitivity of a diagnostic test is expressed as the probability (as a percentage) that a sample tests positive given that the patient has the disease.
The following equation is used to calculate a test’s sensitivity:
Sensitivity = Number of true positives
(Number of true positives + Number of false negatives)
= Number of true positives
Total number of individuals with the illness
The specificity of a test is expressed as the probability (as a percentage) that a test returns a negative result given that the that patient does not have the disease.
The following equation is used to calculate a test’s specificity:
Specificity = Number of true negatives
(Number of true negatives + number of false positives)
= Number of true negatives
Total number of individuals without the illness
Sensitivity vs specificity example
You have a new diagnostic test that you want to evaluate. You have a panel of validation samples where you know for certain whether they are definitely from diseased or healthy individuals for the condition you are testing for. Your sample panel consists of 150 positives and 400 negatives.
After running the samples through the assay, you compare your results to their known disease status and find:
True positives (test result positive and is genuinely positive) = 144
False positive (test result positive but is actually negative) = 12
True negatives (test result negative and is genuinely negative) = 388
False negative (test result negative but is actually positive) = 6
Or, displayed in a contingency table:

Genuinely Positive

Genuinely Negative

Row Total

Test Positive

144

12 
156 
Test Negative

6 
388 
394 
Column Total

150 
400 
550 
Sensitivity = 144 / (144 + 6)
= 144 / 150
= 0.96
= 96 % sensitive
Specificity = 388 / (388 + 12)
= 388 / 400
= 0.97
= 97 % specific
For further information on sensitivity and specificity read the full article here
NOTE: Fill in the four values to calculate the sensitivity and specificity of your test. Click here to learn more about the sensitivity and specificity calculator.