We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

The Isolation and Characterization of Brain Metastasis – Competent Breast Cancer CTCs

Video   May 02, 2013

 

About the Speaker

Dario Marchetti is Professor in the Department of Pathology & Immunology and Director of the CTC Core at Baylor College of Medicine. Dr. Marchetti’s bibliography includes 145 publications, numerous awards, and uninterrupted funding since 1991. He acts as Reviewer of the most relevant journals in cancer research, and on Editorial Boards of “Cancer Microenvironment, “Tumor Viruses”, “Vascular Cell”, “Cancer Letters”, and “Journal of Cellular Biochemistry”, among others. He serves on grant reviewing panels of the National Institutes of Health of USA and Italy, the U.S. Department of Defense, and is a grant Reviewer for other national and international Agencies.AbstractMechanisms of fatal brain metastatic breast cancer (BMBC) are largely unknown.  Similarly, properties and biomarker identification of circulating tumor cells (CTCs), the “seeds” of metastasis, remain elusive.  Here we report novel strategies investigating CTCs isolated from peripheral blood mononuclear cells (PBMCs) of patients with BMBC, including the development and characterization of CTC lines.  We identified a unique BMBC CTC signature (HER2+/EGFR+/HPSE+/Notch1+/EpCAM-) by investigating CTCs that could not be captured by the FDA-approved Veridex CellSearchTM platform (EpCAM - negative CTCs). Second, we analyzed the invasive and metastatic competencies of isolated CTCs.  Established CTC lines over-expressing the BMBC signature were highly invasive and capable to form brain metastasis in xenografts.  Third, tumor cell morphologies of CTC - induced metastases closely resembled those of pathologically assessed tumors of patients whose blood was source of isolated CTCs.  Fourth, the expression of proteins of the BMBC signature was detected in CTC - induced BMBC.  Collectively, we provide first-time evidence of human CTCs isolation and long-term growth, the establishment of CTC lines, and CTC metastatic competency in the presence of a biomarker signature necessary to promote BMBC.

 
 
 
 

Recommended Videos

3D Bioprinting: Could We Study an Asthma Attack in a Dish?

Video

Dr. Sam Wadsworth discusses how 3D bioprinting of organs could assist the drug development process and improve healthcare.

WATCH NOW

The Future of Lab-Grown Eye Transplants

Video

The future of lab-grown eye transplants is... eye-opening. Dr Mariya Moosajee discusses the role of stem cell research in ophthalmology, and the exciting possibilities which lie ahead.

WATCH NOW

Breakthrough in Growing Human Embryos

Video

Growing embryos in vitro for longer allows researchers to tackle major questions in embryology, such as how cells coordinate themselves to achieve major milestones. Cambridge University Professor Magdalena Zernicka-Goetz explains what it means to study embryos at a time when many pregnancies fail.

WATCH NOW

 

Like what you just watched? You can find similar content on the communities below.

Cancer Research

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE