Tangential flow filtration (TFF), also known as cross-flow filtration, is a rapid and efficient method for the separation and purification of biomolecules.

TFF can be used to concentrate sample solutions ranging in volumes from 10 mL to thousands of liters. It can also be used to fractionate large from small biomolecules, harvest cell suspensions and clarify fermentation broths and cell lysates.

Another major usage of TFF is to perform a process called diafiltration, a technique that uses ultrafiltration membranes to completely remove, replace or lower the concentration of salts or solvents from solutions containing proteins, peptides, nucleic acids and other biomolecules.

This webinar will explore the applications of diafiltration across immunology, protein chemistry, molecular biology, biochemistry and biotechnology.

Traditional organoid culture can present challenges for the downstream analysis of single organoids. In this webinar, Dr. Allysa Stern will discuss a unique workflow that enables clonal organoid development, monitoring of iPSC differentiation over time and automated isolation of single organoids.

Dr. Scott Magness will then present a case related to his group’s work investigating tumor cell heterogeneity through clonal organoid morphology and transcriptomics. He will discuss new approaches using single organoid transcriptomics to evaluate organoids derived from single cells from gastric dysplastic tissues and how this approach might reveal new ways to investigate tumor cell heterogeneity and evasion of some cells from cancer treatments.

In this webinar, our expert speaker Dr. Ceri Fielding, from the division of infection and immunity at Cardiff University, will discuss the need to understand how viruses interact with NK cells during the development of novel antiviral therapies.

Modern gene editing technologies continue to transform biological research and push the boundaries of what is possible in and out of the lab. This free-to-attend event will provide industry and academic researchers with a platform to discuss some of the most exciting ways gene editing technologies are being deployed to address real-world challenges in areas spanning human health, agriculture, and climate change.

CRISPR/Cas9, a ground-breaking tool for altering genes with the potential to fully cure genetic diseases, has made its way into the clinic, accelerating research on new therapies using this technology.

This Technology Networks event features exclusive talks focused on the most recent and innovative developments in the world of cell and gene therapies. Our expert speakers will highlight the opportunities cell and gene therapies provide for the treatment of disease and injury, including how cell therapies are providing novel solutions for rare diseases such as Duchenne muscular dystrophy.