Accurate Cytotoxicity and Proliferation Determination: Advantages of a High-throughput Phenotypic Approach Over ATP-luminescence Assays

Here we show the development and implementation of a costeffective,
no-wash HCA assay to simultaneously report the cell
number, percentage of live cells and cell cycle phase distribution
as markers of proliferation and viability. We demonstrate that
this assay can be applied to high density plate formats, and
be imaged and analysed in only 8 minutes per plate using the
acumen Cellista laser scanning imaging cytometer from TTP
Labtech. By comparing the drug-responses of several well characterised
anti-cancer drugs on HeLa cells, we highlight
the key differences between a phenotypic assay enabled by
acumen, and a biochemical ATP-luminescence approach.