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Cell Viability in 3D Culture using in situ Bioluminescence

The paradigm shift from two-dimensional (2D) to 3D cell culture techniques1 has grown rapidly with a 153% increase in 3D cell culture research publications from 2007 to 2008.2 As compared to 2D, 3D culture conditions help organize cells into tissue-like structures that are more similar in form and function.3 Oncology researchers are actively pursuing 3D cell culture techniques to gain the benefits of this more biologically-relevant environment in drug discovery and development, but barriers of lack of a universal and cost-effective 3D culture system have prevented widespread adoption of 3D techniques.