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In Vitro Differentiation of Human iPS Cells into Colon Organoids in Serum-free Cell Culture Conditions

Organoids are complex self-organized 3D cell culture models that are often derived from stem cells. Epithelial intestinal organoids, or “mini-guts”, maintain the physiological characteristics of the gastrointestinal system and are a useful tool to model intestinal development and disease.

Traditional isolation techniques rely on lengthy primary tissue isolation, either from mouse or from difficult-to-source human tissue samples. However, induced pluripotent stem cell-derived organoids would enable the rapid generation of patient-specific cell models from a wider range of human donors.

Download this whitepaper to discover a human iPSC-derived colon organoid system that, in just three steps, is:

  • Highly characterized
  • Assay-ready
  • Cryopreserved