In Vitro Differentiation of Human iPS Cells into Colon Organoids in Serum-free Cell Culture Conditions
Organoids are complex self-organized 3D cell culture models that are often derived from stem cells. Epithelial intestinal organoids, or “mini-guts”, maintain the physiological characteristics of the gastrointestinal system and are a useful tool to model intestinal development and disease.
Traditional isolation techniques rely on lengthy primary tissue isolation, either from mouse or from difficult-to-source human tissue samples. However, induced pluripotent stem cell-derived organoids would enable the rapid generation of patient-specific cell models from a wider range of human donors.
Download this whitepaper to discover a human iPSC-derived colon organoid system that, in just three steps, is:
- Highly characterized