Performance Characterization of Batch Fed Non-Adherent CHO Cell Culture in Rocker Bags

Disposable cell culture bags are used in conjunction with rocker platforms in an array of cultivation applications (insect and mammalian cell lines, virus, protein expression). Rocking platforms provide the dynamic motion and oxygen transfer for cell culture environments. Disposable culture bags are designed to allow for a large air-liquid surface for oxygen transfer, easy access for sampling/filling, and a sterile environment. Venting issues have been reported in the field during rocking of disposable cell culture bags. Therefore, venting and cell growth testing were performed for optimized Charter Medical Clear-Pak® Cell Culture bags.

Data collected from the testing provide evidence that the Charter Clear-Pak® cell culture bags produced optimal performance for the cultivation of non-adherent CHO animal cells.