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Latest App Notes & Case Studies

App Note / Case Study

Sectioning of Bone as a Specialist Histology Specimen

This application note discusses how the Thermo Scientific HM355S and Finesse ME+ Automated Microtomes deliver quality sections from bone tissue.
App Note / Case Study

Proteomic Analysis of Laser Microdissected FFPE Tissue Using Mass Spectrometry

This application note discusses a study which was undertaken to identify candidate protein biomarkers for early detection of endometrial cancer using formalin fixed paraffin embedded tissue samples.
App Note / Case Study

Mass Spec Protein Assays in FFPE Tissue

This application note discusses how the Liquid Tissue®-SRM platform makes possible quantitation of proteins and their phosphorylation states by mass spectrometry using small amounts of laser microdissected FFPE tissue.
App Note / Case Study

Triggering with the Leica TCS SP5, TCS SP5 II and DM6000 CFS

This document provides detailed information about triggering with the Leica TCS SP5, TCS SP5 II and DM6000 CFS systems.
App Note / Case Study

On-screen Analysis: Review Captured Cells on-Screen

This application note shows the methodology for viewing and evaluating captured cells on screen.
App Note / Case Study

Sample preparation with the AC Extraction Plate: extraction of urinary THC metabolites for LC-MSMS quantification

In many countries, routine workplace drug testing involves screening for the free THC-COOH metabolite obtained by hydrolyzing THC-COOH glucuronide. Tecan’s AC Extraction Plate with TICE technology enables a straightforward workflow that is reproducible and cost efficient. Liquid chromatography coupled with tandem mass spectrometry (LC-MSMS) provides an easy and cost-effective means for fast, sensitive and precise analysis of THC and its metabolites.
App Note / Case Study

Automated sample preparation for the determination of testosterone in serum by LC-MSMS

For accurate, reproducible quantification of testosterone, a growing number of laboratories are shifting from immunoassay to liquid chromatography coupled with tandem mass spectrometry. This application note describes the preparation of serum samples for subsequent LC-MS analysis of testosterone, focusing on a streamlined workflow suitable for automation.
App Note / Case Study

Validating the Expression Consistency of a Housekeeping Protein

Housekeeping proteins (HKP) are typically used as loading controls because it is assumed that the HKP expression level remains consistent across samples. However, there is evidence that HKP expression levels change in many scenarios, including siRNA treatment, cell death, cell differentiation, etc.
App Note / Case Study

A Method for Greater Reliability in Western Blot Loading Controls: Stain-Free Total Protein Quantitation

Reliable assessment of changes in target protein levels by western blot requires measurement of both the target and loading control proteins in the linear dynamic range. Stain-free technology is a novel method introduced by Bio-Rad to visualize and quantify proteins in gels and blots.
App Note / Case Study

General V3 Western Blotting Protocol

Western blotting is a very useful and widely adopted lab technique, but the traditional procedure can be long and tedious. Researchers can assess only whether a blot image is captured at the end of the long procedure and the quality of western blot data is sometimes challenged due to poor loading controls.
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