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Latest App Notes & Case Studies

App Note / Case Study

A Method for Greater Reliability in Western Blot Loading Controls: Stain-Free Total Protein Quantitation

Reliable assessment of changes in target protein levels by western blot requires measurement of both the target and loading control proteins in the linear dynamic range. Stain-free technology is a novel method introduced by Bio-Rad to visualize and quantify proteins in gels and blots.
App Note / Case Study

General V3 Western Blotting Protocol

Western blotting is a very useful and widely adopted lab technique, but the traditional procedure can be long and tedious. Researchers can assess only whether a blot image is captured at the end of the long procedure and the quality of western blot data is sometimes challenged due to poor loading controls.
App Note / Case Study

Determining the Appropriate Sample Load When Using a Stain-Free V3 Western Workflow™

Traditional western blot workflows require an appropriate sample load to detect both target proteins and loading control proteins. The discrepancy between these quantities often leads to oversaturated protein bands, forcing researchers to sometimes run two duplicate gels or titrate down the antibody concentration in order to get quantitative results.
App Note / Case Study

Determining the Appropriate Sample Load for Western Blots

Reliable western blot data can be generated only when the proper sample amount of protein is used. Loading too much protein leads to signal saturation in western blots, yet too little produces weak signals.
App Note / Case Study

Avoiding Housekeeping Protein Detection Saturation

Reliable western blot data require detection of the target and loading control proteins in the linear dynamic range. Many published western blot images show saturated protein bands, indicating that they were not measured in the linear dynamic range. Quantitative analysis based on this type of data is not reliable.
App Note / Case Study

Purification of Genomic DNA from Saliva Using Oragene® Discover Collection Kits and the Akonni TruTip® Extraction System on the Hamilton Microlab® STAR Liquid Handling Workstation

In the following studies, we have demonstrated the high precision of the automated TruTip extraction technology in processing genomic DNA from low-volume saliva samples and its advantage over competitors.
App Note / Case Study

Next Generation Sequencing Method for Illumina TruSeq DNA Sample Preparation Protocol on the Hamilton STAR

The purpose of the TruSeq sample preparation protocol is to add adapter sequences onto the ends of DNA fragments to generate multiplexed sequencing libraries. Good liquid handling is crucial for pipetting precise volumes for example for Agilent Bioanalyzer.
App Note / Case Study

Automated Liquid-liquid Extraction of 25-Hydroxy Vitamin D from Serum and Preparation for LC/MC Analysis Using the Hamilton Robotics Microlab STARlet Workstation

This application note demonstrates the automation capabilities of Hamilton Robotics’ Microlab STARlet and how it can be used to prepare clinical serum samples for Vitamin D analysis quickly, reliably and cost effectively.
App Note / Case Study

Recombinase Polymerase Amplification

This application note explains the advantages of RPA and how it simplifies the engineering challenges for point-of-care diagnostics.

App Note / Case Study

Quantitative Determination of Total Bile Acids with a Thermo Scientific Varioskan Flash Multimode Reader Using a Kinetic Enzyme Cycling Assay

Serum total bile acids (TBA) level is a long-established and accepted indicator of liver diseases. This application note presents a simple photometric measurement of serum TBA.
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