Latest App Notes & Case Studies

Reliable assessment of changes in target protein levels by western blot requires measurement of both the target and loading control proteins in the linear dynamic range. Stain-free technology is a novel method introduced by Bio-Rad to visualize and quantify proteins in gels and blots.

Western blotting is a very useful and widely adopted lab technique, but the traditional procedure can be long and tedious. Researchers can assess only whether a blot image is captured at the end of the long procedure and the quality of western blot data is sometimes challenged due to poor loading controls.

Traditional western blot workflows require an appropriate sample load to detect both target proteins and loading control proteins. The discrepancy between these quantities often leads to oversaturated protein bands, forcing researchers to sometimes run two duplicate gels or titrate down the antibody concentration in order to get quantitative results.

Reliable western blot data can be generated only when the proper sample amount of protein is used. Loading too much protein leads to signal saturation in western blots, yet too little produces weak signals.

Reliable western blot data require detection of the target and loading control proteins in the linear dynamic range. Many published western blot images show saturated protein bands, indicating that they were not measured in the linear dynamic range. Quantitative analysis based on this type of data is not reliable.

In the following studies, we have demonstrated the high precision of the automated TruTip extraction technology in processing genomic DNA from low-volume saliva samples and its advantage over competitors.

The purpose of the TruSeq sample preparation protocol is to add adapter sequences onto the ends of DNA fragments to generate multiplexed sequencing libraries. Good liquid handling is crucial for pipetting precise volumes for example for Agilent Bioanalyzer.

This application note demonstrates the automation capabilities of Hamilton Robotics’ Microlab STARlet and how it can be used to prepare clinical serum samples for Vitamin D analysis quickly, reliably and cost effectively.

This application note explains the advantages of RPA and how it simplifies the engineering challenges for point-of-care diagnostics.

Serum total bile acids (TBA) level is a long-established and accepted indicator of liver diseases. This application note presents a simple photometric measurement of serum TBA.