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Latest Application Notes

Application Note

On-screen Analysis: Review Captured Cells on-Screen

This application note shows the methodology for viewing and evaluating captured cells on screen.
Application Note

Sample preparation with the AC Extraction Plate: extraction of urinary THC metabolites for LC-MSMS quantification

In many countries, routine workplace drug testing involves screening for the free THC-COOH metabolite obtained by hydrolyzing THC-COOH glucuronide. Tecan’s AC Extraction Plate with TICE technology enables a straightforward workflow that is reproducible and cost efficient. Liquid chromatography coupled with tandem mass spectrometry (LC-MSMS) provides an easy and cost-effective means for fast, sensitive and precise analysis of THC and its metabolites.
Application Note

Automated sample preparation for the determination of testosterone in serum by LC-MSMS

For accurate, reproducible quantification of testosterone, a growing number of laboratories are shifting from immunoassay to liquid chromatography coupled with tandem mass spectrometry. This application note describes the preparation of serum samples for subsequent LC-MS analysis of testosterone, focusing on a streamlined workflow suitable for automation.
Application Note

Validating the Expression Consistency of a Housekeeping Protein

Housekeeping proteins (HKP) are typically used as loading controls because it is assumed that the HKP expression level remains consistent across samples. However, there is evidence that HKP expression levels change in many scenarios, including siRNA treatment, cell death, cell differentiation, etc.
Application Note

A Method for Greater Reliability in Western Blot Loading Controls: Stain-Free Total Protein Quantitation

Reliable assessment of changes in target protein levels by western blot requires measurement of both the target and loading control proteins in the linear dynamic range. Stain-free technology is a novel method introduced by Bio-Rad to visualize and quantify proteins in gels and blots.
Application Note

General V3 Western Blotting Protocol

Western blotting is a very useful and widely adopted lab technique, but the traditional procedure can be long and tedious. Researchers can assess only whether a blot image is captured at the end of the long procedure and the quality of western blot data is sometimes challenged due to poor loading controls.
Application Note

Determining the Appropriate Sample Load When Using a Stain-Free V3 Western Workflow™

Traditional western blot workflows require an appropriate sample load to detect both target proteins and loading control proteins. The discrepancy between these quantities often leads to oversaturated protein bands, forcing researchers to sometimes run two duplicate gels or titrate down the antibody concentration in order to get quantitative results.
Application Note

Determining the Appropriate Sample Load for Western Blots

Reliable western blot data can be generated only when the proper sample amount of protein is used. Loading too much protein leads to signal saturation in western blots, yet too little produces weak signals.
Application Note

Avoiding Housekeeping Protein Detection Saturation

Reliable western blot data require detection of the target and loading control proteins in the linear dynamic range. Many published western blot images show saturated protein bands, indicating that they were not measured in the linear dynamic range. Quantitative analysis based on this type of data is not reliable.
Application Note

Purification of Genomic DNA from Saliva Using Oragene® Discover Collection Kits and the Akonni TruTip® Extraction System on the Hamilton Microlab® STAR Liquid Handling Workstation

In the following studies, we have demonstrated the high precision of the automated TruTip extraction technology in processing genomic DNA from low-volume saliva samples and its advantage over competitors.
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