Flow Cytometry Protocols for Extracellular and Intracellular Targets
Flow cytometry is a high throughput quantitative application that employs fluorescent-labeled molecules for multi-parametric analysis of millions of individual cells or particles, providing information about the size/granularity of the cells.
However, with specific needs for each experiment and the addition of sorting, mass spectrometry and imaging, the field of flow cytometry is becoming more and more complex.
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A schematic overview of a flow cytometry analysis
Benchtop protocols for surface staining in flow cytometry
Examples of flow cytometry data