Proteomic Analysis of Laser Microdissected FFPE Tissue Using Mass Spectrometry

Liquid Tissue® lysates were prepared from microdissected epithelial cells obtained from both early stage endometrial cancer and normal endometrium. Global mass spectrometry profiling of all lysates followed by spectral count quantitation indicate differential expression of proteins that include known cancer biomarkers; low-abundance proteins such as transcription factors and signal pathway proteins; and housekeeping proteins. These differentially expressed proteins also include candidate protein biomarkers of early stage endometrial cancer which can be utilized as improveddiagnostic, prognostic, and therapeutic targets.