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Quantitative Profiling of Cell Type Composition in Solid Tissue Samples

Testube for solid Tissue Samples
Credit: iStock

When studying cellular heterogeneity and immune composition within the tumor microenvironment, traditional cell profiling methods have been limited by  the number of markers they can interrogate.

Now, digital software suites can be used to profile the cellular composition of tissues from bulk RNA-sequencing (RNA-seq) data. In particular, complex tissue types such as fresh/frozen (FF) or formalin-fixed paraffin-embedded (FFPE) samples require specialized methods to remove noise and technical variation.

This application note explores the performance characteristics of a NGS targeted enrichment panel for profiling both FF and FFPE tissue samples using a state-of-the-art iSort software solution for digital cytometry.

Download this application note to learn more about:

  • Efficient and reliable enumeration of cell types from FF or FFPE tissues
  • Target enrichment for targeted RNA sequencing
  • Software to quantify cellular composition from bulk tissue expression data

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