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Streamlined RNA Capture for Gene Expression and Novel Fusions

Detecting Coding Genes
Credit: Twistbio

While total RNA sequencing (RNA-seq) offers an impartial glimpse into cellular transcription, analysis can be overcomplicated in the presence of highly abundant non-coding transcripts such as ribosomal RNA, intronic sequences originating from pre-mRNA, and potential genomic DNA contaminants. 

This application note presents capture sequencing using a novel RNA exome panel as a powerful approach to specifically target the transcriptome to profile gene expression and discover novel fusion genes.

Download this app note to learn more about:

  • The “exon-aware” design strategy
  • How exome capture compares to whole transcriptome sequencing and 3’-counting
  • Targeted RNA sequencing on low-mass inputs and FFPE samples 

Download this App Note for FREE Below
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