Using Electron Activated Dissociation (EAD) To Characterize Challenging Metabolites

Glucuronidation is an essential metabolic pathway for drug clearance and its characterization is crucial for optimizing the efficiency and safety of drug candidates.

However, the identification and characterization of glucuronide-drugs conjugates can be challenging using by MS/MS alone, as the glucuronic acid bond is often labile.

This application note presents a quick and robust soft-spot identification procedure using diagnostic fragments from electron activated dissociation (EAD).

Download this app note to discover how EAD:

• Enhances the sensitivity of MS/MS workflows
• Enables a comprehensive characterization of critical glucuronide metabolites
• Helps to implement a streamlined data processing