Advancing Live Cell Analysis
Advancing Live Cell Analysis
Complete the form below and we will email you a PDF version of "Advancing Live Cell Analysis"
We spoke to Tracey Zimmermann, VP of Global Sales at Phasefocus to learn more about the application areas for Livecyte™ and what unique new insights the technology will enable researchers and drug developers to reveal about the behaviour of cells’
TZ: Livecyte is a unique imaging system for live cell analysis which uses an optimized version of Quantitative Phase Imaging called Ptychography (tie-cog-rafee), to enable Kinetic Cytometry: the automatic tracking and analysis of phenotypic and kinetic behaviour of individual cells and cell populations over hours or days. The system produces high contrast, high fidelity images which are artefact free, quantitative and can be tracked with ease across the whole time-course, without the need for cell labelling or high intensity light imaging. Since the power of the illumination laser is thousands of times less than that used for traditional fluorescence light microscopy, cells suffer far less phototoxic shock-a particular advantage for fragile cells such as stem cells or primary cells.
JR: What challenges will this new system help researchers to overcome?
Another challenge facing time-course assays can be focal drift which can affect image quality, which in turn makes it very difficult to track cells. Livecyte’s ptychographic imaging technique ensures that cells are always in focus and since the system delivers a continuous field-of-view which is arbitrarily large (and can be up to a few millimetres in a single image), the need to stitch images is removed. Additionally, imaging continuously over multiple regions (at different magnifications if desired), within a single well of a multi-well plate or multiple regions within each well of a multi-well plate, ensures maximum productivity from each experiment.
JR: Phasefocus recently had a paper published in Nature Scientific Reports validating this new technology. Could you tell us about the significance of the results the paper described?
TZ: The paper was published in conjunction with researchers from University of York and highlights the power of ptychography for long-term imaging of complex primary neuronal cultures in conditions which are effectively “closer to real life”: http://www.nature.com/articles/srep22032. The paper reveals the key advantages to a researcher that a ptychographic imaging approach can bring. The non-invasive properties of the technique were demonstrated by observing the maturation of primary hippocampal cultures into neurons with extensive neurite connectivity, together with the proliferation of microglia and astrocytes. Quantitative phase time-lapse movies of cells within the three populations were generated, which can be viewed alongside the publication. Crucially, since quantitative data was available on every single cell, motility metrics such as speed, meandering index and Euclidean distance could be calculated and these could be linked with changes in morphology, thus deepening understanding of the behaviour of individual cells over time. In this paper, (which used VL21, the Livecyte predecessor), cell tracking was carried out manually using Image J, but using Livecyte, powerful tracking software (patent pending) allows tracking of cells automatically rather than the manual approach employed here.
JR: Livecyte allows researchers to interleave fluorescence measurements during different drug treatments or across cell types within a single experiment. Why is this an important step forward?