Cell Culture Solutions for Biomanufacturing

CHO Playbook Gibco™ CHO cell culture solutions for biomanufacturing 2 Foreword Optimizing process productivity, improving scalability, and increasing efficiency are central to developing a successful biopharmaceutical manufacturing process. To help you improve performance and maintain consistent product quality, Thermo Fisher Scientific offers a broad portfolio of high-performance CHO media, feeds, supplements, cell line development kits, and media manufacturing and optimizing services. CHO solution process flow chart 3 You own your cell culture media formulations You are interested in developing a new CHO cell line You are interested in evaluating catalog media Good outcome Further evaluation: different concentrations or culture conditions Good outcome Good outcome Good outcome Further evaluation and technical advice by FAS Further development Explore our wide range of products for your process development NO YES YES YES NO YES NO NO NO Customer Owned Formulation NO You are interested in Gibco media panel evaluations or media development services You are interested in cell line development services Cell Line Development Services Freedom Cell Line Development Kits YES NO YES Looking for more support? Watch our Cell Culture Media Selection and Manufacturing Journey Webinar to learn how to accelerate time to market and increase production. Watch the webinar CHO catalog media, feed, and supplement navigation guide 4 Identify your cell line: Peptones for CHO cell culture applications Tour our facilities virtually Select your Gibco™ medium: Select your Gibco™ feed: Predictably and accurately achieve a targeted glycan profile: Boost your titer by adding a Gibco™ supplement: DynamisTM Medium FunctionMAXTM TiterEnhancer Perfusion process: Gibco™ High-Intensity Perfusion (HIP) CHO Medium CD CHO Medium CD OptiCHOTM Medium Efficient-ProTM Feed 1 Efficient-ProTM Feed 2 ExpiCHOTM Stable Production Medium (SPM) ExpiCHO-S ExpiCHO-S GS CHO Other CHO GS CHO CHO-S CHO-S DXB11 DG44 CHO-K1 GlycanTuneTM C+ Total Feed EfficientFeedTM B+ Supplement EfficientFeedTM C+ Supplement EfficientFeedTM A+ Supplement GlycanTuneTM B+ Total Feed GlycanTuneTM A+ Total Feed Efficient-ProTM Medium CHO-S/DG44/GS Efficient-ProTM Medium CHO-K1/GS Spotlight on perfusion • Savings that speak volumes - the economic benefits of perfusion bioprocessing. • Perfusion medium considerations. Learn more in the white paper Discover more in the ebook Watch the video • Gibco perfusion medium. Description of CHO (Chinese hamster ovary) cells 5 Peptones for CHO cell culture applications Tour our facilities virtually • Predominant host cell line for stable expression in large-scale production and manufacturing of biotherapeutic proteins • Robust, scalable, and high titer • Enables various post-translational modifications • Well-adapted to suspension culture and can grow to high densities, particularly in bioreactors • Safe production platform (low human virus susceptibility) • Some proteins that are difficult to express in HEK293 cells may be expressed better in CHO cells • Common variants for biomanufacturing CHO-K1, CHO DG44, CHO-S, CHO-GS, etc. • Different CHO clones often possess diverse nutritional requirements that are unique to each clone CHO Cells (Chinese hamster ovary cells) Could cell line development kits help enhance your CHO processes? Learn how the Freedom ExpiCHO-S kit enhances productivity. Freedom cell line development kits. Discover Gibco™ Freedom™ cell line development kits For cell line development services, please contact us at our website. Learn more here Read the app note Mutation landscape of CHO cell lines 6 Peptones for CHO cell culture applications Tour our facilities virtually Original CHO line (Puck 1957) CHO DG44 (cGMP banked) (Life Technologies, A1097101) CHO Pro- 3 (DHFR+) (Flintoff, 1976) CHO-MTXRIII DHFR mutant CHO-DG44 (DHFR-) (Urlaub & Chasin, 1983) (Avail from L. Chasin) CHO-S (cGMP banked) (Life Technologies, A1136401) CHO-S (Tilkins, 1991) C0101 (Production cell line) CHO-K1 ATCC (Puck, ATCC CCL-61) CHO-K1 ECACC (85051005) CHO-K1/SF (ECACC 93061 607) CHO variant (Tobey, 1962) CHO-K1 (Kao & Puck, 1968) CHO protein free (ECACC 00102307) EMS exposure Gamma rays CHO cell lines CHO DG44 CHO K1 ATCC CHO K1 ECACC CHO Protein Free CHO-K1 /SF CHO-S CO101 7 Peptones for CHO cell culture applications CHO (Chinese hamster ovary) cells Nutritional diversity • Balanced composition to meet specific requirements of all cell types Protective effects • Nutritional buffering • Protects cells from toxic levels of media components • Allows for higher concentrations of key components • Delays onset of apoptosis Peptone selection in CHO cell cultures How to choose the right peptone for CHO cell culture processes Select a good complete base medium • More enriched base media yield better results than deficient media (e.g., CD or SF complete media) Select from a wide variety of peptones to evaluate as supplement and feed • Different substrates (e.g., yeast, soy, cotton, or wheat) • Multiple products from the same substrate Characterize the culture throughout the process using analytical techniques • Establish baseline data using spent media in multiple runs with multiple lots of peptones (e.g., feed strategy: timing and concentration of feed addition) Evaluate performance on proliferation, production, and protein quality • Monitoring only one attribute does not always predict overall performance (e.g., glycosylation and charge variant profiles) Create a thorough experimental design • Multiple concentrations (e.g., 1g/L, 3g/L, 5g/L, 7g/L, and 9g/L) • Individual and blended conditions (e.g., blend of yeast and soy peptones) Tour our facilities virtually Components of a typical peptone 25% 10% 15% 20% Polypeptides 30% Free amino acids Carbohydrates Salt and trace metals Other components (vitamins, organic acids, organic nitrogen bases, etc.) Adding the right peptone to your process can dramatically improve the culture environment to achieve production goals. Learn more about how to screen and select the right peptone for your process. Watch the video 8 Gibco Peptone Starter Packs Tour our facilities virtually Get Started With the GibcoTM Starter Paks Product Description Contents Starter Pak No. 1 Ultra-filtered peptones ideal for human health applications DifcoTM Yeast Extract, UF BactoTM TC Yeastolate DifcoTM TC Yeastolate, UF DifcoTM Phytone Supplement, UF BactoTM Yeast Extract, Technical Starter Pak No. 2 Animal origin–free and animal origin peptones best suited for vaccine production BactoTM Yeast Extract DifcoTM Soytone PhytoneTM Peptone BactoTM Proteose Peptone No. 2 BactoTM Proteose Peptone No. 3 BactoTM Casamino Acids Starter Pak No. 3 Animal origin–free peptones for animal and human vaccine production BactoTM Malt Extract BactoTM Yeast Extract Yeast Extract BactoTM TC Yeastolate PhytoneTM Peptone DifcoTM Soytone Preview Pak Provide new peptones with unique nutritional profiles, suitable for mammalian and microbial applications Wheat 100 UF Cotton 100 UF Cotton 200 UF Soy 100 Yeast 100 Click on the product name to open the product details page. Looking for more information to support your peptone selection? Technical guide to peptones, supplements, and feeds. Discover how peptones can increase titer and optimize productivity. Using peptones to achieve demanding bioproduction goals. Read more here Watch the webinar Read the case study 9 Gibco Media and Feed Panel Evaluations CHO media and feed panels Testing a broad range of formulations increases the possibility that you will be able to zero in on a nutrient composition that best supports your clone’s specific needs. The GibcoTM Media Panel Evaluation was developed with diversity in mind, providing you with a panel of products that each have distinct formulations and nutrient levels. This can enable you to quickly identify the components driving your increases in productivity and quality. What’s included in a panel evaluation? 500 mL bottles of ready-to-use media and feed formulations Detailed protocol Consultation by FAS and R&D teams Data analysis and report Clone Nutritional Needs Formula #1 Formula #3 Formula #4 Formula #5 Formula #2 Peptones for CHO cell culture applications Tour our facilities virtually Could panels benefit your media development process? We spoke to Thermo Fisher Scientific media and feed panel specialists to learn more. Read the Q&A here 10 Gibco Media and Feed Panel Evaluations Discover the diversity you can find in our Gibco panels Peptones for CHO cell culture applications Tour our facilities virtually Gibco CHO Feed Panel Medium #1 Medium #2 Medium #3 Medium #4 Medium #5 AMINO ACIDS VITAMINS LIPIDS TRACE METALS AMINES NUCLEO SIDES ORGANIC ACIDS Gibco CHO Media Panel Components Medium #1 Medium #2 Medium #3 Medium #4 Medium #5 Medium #6 Medium #9A Medium #13 Medium #14 AMINO ACIDS VITAMINS LIPIDS TRACE METALS AMINES NUCLEOSIDES ORGANIC ACIDS Please visit our website on CHO media and feed panels to learn more. High Level Low Level Rapid Media and Feed Screening Options for Targeted CHO Clones. Watch the webinar 11 Media development services Why choose Thermo Fisher for media development? Optimize your unique medium and process for increased titers, quality, and manufacturability by partnering with GibcoTM Media by DesignTM Services. Our flexible media development options include traditional workflow, multiomics workflow, and key driver identification1 (KDI) workflow. The multi-omics option incorporates insights from our analyses of thousands of components (proteins and metabolites) within the cell, in addition to spent media analysis, to more fully characterize the nutritional requirements of your cell line. Experience and expertise • More than 60 years of cell line / media development experience • 160+ projects completed since 2006 • Decades of tech transfer expertise • Offering best-in-class technology to support our data intelligence, including high resolution UPLC-MS instrumentation and predictive analytics Collaborative • Worked with >100 client process development groups • 35% of projects from repeat customers • Dedicated business team, support team, and program managers Consistency • World-class processes and facilities • Assurance of supply to meet demands • Scalability to GMP • Rapid Prototyping Service Peptones for CHO cell culture applications Tour our facilities virtually 1. Key driver identification (KDI) workflow: Our KDI approach uses statistical modeling of analytical data generated to identify critical media components that may have significant impacts on process performance. These components must be kept within specific concentration ranges to achieve optimal and consistent results. This model is then used to inform media design to achieve improved productivity and enhanced consistency. Discover the benefits of working with a third-party custom media development collaborator. Are you considering outsourcing your media development? Learn more in the article 12 Media development services Peptones for CHO cell culture applications Tour our facilities virtually Traditional workflow option Our traditional development workflow uses spent media analyses of key components, including selected water-soluble vitamins and amino acids. These insights are used in an iterative design of experiments (DOE) process to develop optimized formulations. Multi-omics workflow option Combining proteomics and metabolomics, our multi-omics workflow employs cellular component analyses of thousands of proteins and metabolites to fully understand the nutritional requirements of your cell line. This results in a final formulation that can help you: • Increase titers • Enhance protein quality • Improve productivity and cost efficiencies Traditional workflow. A typical full traditional optimization project workflow follows these phases and evaluates up to 50 molecules. Multi-omics workflow. A typical multi-omics optimization project involves these phases and can evaluate up to 1,000 metabolites and 7,000 proteins. Please visit our website on media development services to learn more. Could multi-omics enhance your production and maximize your cell line potential? Learn more about the Gibco multi-omics difference. Custom traditional and multi-omics media development enhances antibody production in CHO cells. Advances in custom media development: the multi-omics difference. Read the brochure Read the case study Watch the video 13 Product list for CHO cells Chinese hamster ovary (CHO) media Peptones for CHO cell culture applications Tour our facilities virtually Gibco product and format Quantity Gibco optimized cell lines Gibco recommended feeds and supplements Features Cat. No. CD DG44 Medium, liquid 1,000 mL DG44, DXB11, and other DHFR– cell lines Once transfected, scale-up is recommended in CD OptiCHO™ ™ A+ Supplement • Optimized for the growth of dihydrofolate reductase– – ) cells in suspension, contains hypoxanthine and thymidine (HT), without surfactant • Also available in Gibco™ Freedom™ DG44 Kit with cGMP-banked cells (Cat. No. A13737-01) 12610-010 Freedom™ CHO-S Kit – Kit contains CHO-S™ cells (cGMP-banked) and media ™ C+ Supplement • Easy-to-use, beginning-to-end product, for cloning and expression of recombinant proteins in CHOderived suspension culture • The Freedom CHO-S Kit includes components for transfection, expression, clone creation, and stable cell line selection, all in a conveniently packaged kit A1369601 Freedom™ DG44 Kit – Kit contains DG44 cells (cGMP-banked) and media NA • Easy-to-use, beginning-to-end product, for cloning and expression of recombinant proteins in DHFR– CHO cells • The Freedom DG44 Kit includes components for transfection, expression, clone creation, and stable cell line selection, all in a conveniently packaged kit A1373701 CD CHO Medium, liquid 500 mL CHO-S and glutamine synthetase (GS) CHO ™ B+ Supplement, GlycanTune™ B+ Total Feed, GlycanTune™ Feed Kit B+ • Best with past culture success in CD CHO cells, and 10743-011 1,000 mL 10743-029 10 L BPC 10743-001 20 L BPC 10743-002 CD CHO AGT Medium, granulated powder 1 L NA • Also available: CHO-S cells (cGMP-banked) and Media Kit (Cat. No. A11557-01) 12490-017 10 L 12490-025 100 L 12490-001 10 kg 12490-003 CD OptiCHO™ Medium, liquid 1,000 mL DG44, DXB11, and CHO cells except CHO K1, GS CHO, and CHO-S ™ A+ Supplement, GlycanTune™ A+ Total Feed, GlycanTune™ Feed Kit A+ • Lower osmolality formulation when feeding optimization is important and best for hard-to-grow or hard-to-adapt cells 12681-011 6 x 1,000 mL 12681-029 CD OptiCHO AGT Medium, granulated powder 1 L A11222-04 10 L A11222-05 100 L A11222-01 10 kg A11222-03 Dynamis™ Medium, liquid 1,000 mL DG44, CHO K1, GS CHO, and CHO-S ™ C+ Supplement, GlycanTune™ C+ Total Feed, GlycanTune™ Feed Kit C+ • Best for achieving maximal productivity with high cell densities and cell viability at harvest A26615-01 Dynamis AGT Medium, granulated powder 1 L A26175-04 10 L A26175-01 100 L A26175-02 10 kg A26175-03 ExpiCHO™ Stable Production Medium (SPM), liquid 1,000 mL ExpiCHO-S™ cells (cGMP-banked, Cat. No. A37785) GlycanTune C+ Total Feed, GlycanTune Feed Kit C+ • Enables seamless transition from transient to stable production without additional medium optimization • Use ExpiCHO™ Expression Medium (Cat. No. A29100) for transfection A37110-01 ExpiCHO Stable Production AGT Medium, granulated powder 10 L A37111-01 100 L A37111-02 450 L A37111-03 High-Intensity Perfusion CHO Medium (HIP CHO), AGT granulated powder 1 x 2 L DG44, CHO K1, GS CHO, and CHO-S cell lines designed for high-density At full medium concentration, designed to be used without additional feed • Designed to work with all types of suspension continuous perfusion processes • concentrations for both seed train and production work with cell lines that may be sensitive to more concentrated media • Supports high cell densities while reducing medium exchange rates—results from continuous perfusion of >100 million cells/mL at 1 VVD A42302-01 15 x 2 L A42302-02 100 x 2 L A42302-03 370 x 2 L A42302-03 CHO Cell Culture Medium, liquid and dry powder 1,000 mL DG44, CHO-K1, other CHO cell lines, and BHK-21 cells NA • A high-performance, complete formulation yielding cell growth and production of antibodies and recombinant proteins in suspension without the need for further supplementation • Contains Gibco™ Difco™ 220253 10 kg 670006 CD-1000 Medium, liquid and dry powder 1,000 mL CHO, NS0, and hybridoma cells Various hydrolysates or CD supplements • Designed as a platform medium suitable for multiple cell types • Ideally suited for further hydrolysate or chemically and perfusion processes for both research and manufacturing applications 215204 20 L BPC 215205 500 g 215206 10 kg 215207 Easy-Adapt™ CHO-K1 CD Medium, liquid 1,000 mL CHO-K1 and CHO NA • CHO-K1 cells need; rich in carbohydrates, amino acids, and vitamins 670112 20 L BPC 670114 CHO Media Panel 9 x 500 mL Diverse media panel designed to identify optimal formulation for various CHO cell lines CHO catalog feeds or CHO feed panel • Diverse library of formulations • Evaluation protocol • Technical evaluation support • Easy to customize • Scalable to rapid prototyping and GMP Order through Gibco Custom Media Services (gibcopanelteam@ ) ™ HT Supplement (Cat. No. 11067). Note: Express Services, go to For production of recombinant proteins and monoclonal antibodies (mAbs) in CHO suspension cell culture; all are AOF, chemically defined and protein-free without phenol red, glutamine, hydrolysates, hypoxanthine*, or thymidine*, unless noted otherwise. Gibco product and format Quantity Gibco optimized cell lines Gibco recommended feeds and supplements Features Cat. No. CD DG44 Medium, liquid 1,000 mL DG44, DXB11, and other DHFR– cell lines Once transfected, scale-up is recommended in CD OptiCHO™ ™ A+ Supplement • Optimized for the growth of dihydrofolate reductase– – ) cells in suspension, contains hypoxanthine and thymidine (HT), without surfactant • Also available in Gibco™ Freedom™ DG44 Kit with cGMP-banked cells (Cat. No. A13737-01) 12610-010 Freedom™ CHO-S Kit – Kit contains CHO-S™ cells (cGMP-banked) and media ™ C+ Supplement • Easy-to-use, beginning-to-end product, for cloning and expression of recombinant proteins in CHOderived suspension culture • The Freedom CHO-S Kit includes components for transfection, expression, clone creation, and stable cell line selection, all in a conveniently packaged kit A1369601 Freedom™ DG44 Kit – Kit contains DG44 cells (cGMP-banked) and media NA • Easy-to-use, beginning-to-end product, for cloning and expression of recombinant proteins in DHFR– CHO cells • The Freedom DG44 Kit includes components for transfection, expression, clone creation, and stable cell line selection, all in a conveniently packaged kit A1373701 CD CHO Medium, liquid 500 mL CHO-S and glutamine synthetase (GS) CHO ™ B+ Supplement, GlycanTune™ B+ Total Feed, GlycanTune™ Feed Kit B+ • Best with past culture success in CD CHO cells, and 10743-011 1,000 mL 10743-029 10 L BPC 10743-001 20 L BPC 10743-002 CD CHO AGT Medium, granulated powder 1 L NA • Also available: CHO-S cells (cGMP-banked) and Media Kit (Cat. No. A11557-01) 12490-017 10 L 12490-025 100 L 12490-001 10 kg 12490-003 CD OptiCHO™ Medium, liquid 1,000 mL DG44, DXB11, and CHO cells except CHO K1, GS CHO, and CHO-S ™ A+ Supplement, GlycanTune™ A+ Total Feed, GlycanTune™ Feed Kit A+ • Lower osmolality formulation when feeding optimization is important and best for hard-to-grow or hard-to-adapt cells 12681-011 6 x 1,000 mL 12681-029 CD OptiCHO AGT Medium, granulated powder 1 L A11222-04 10 L A11222-05 100 L A11222-01 10 kg A11222-03 Dynamis™ Medium, liquid 1,000 mL DG44, CHO K1, GS CHO, and CHO-S ™ C+ Supplement, GlycanTune™ C+ Total Feed, GlycanTune™ Feed Kit C+ • Best for achieving maximal productivity with high cell densities and cell viability at harvest A26615-01 Dynamis AGT Medium, granulated powder 1 L A26175-04 10 L A26175-01 100 L A26175-02 10 kg A26175-03 ExpiCHO™ Stable Production Medium (SPM), liquid 1,000 mL ExpiCHO-S™ cells (cGMP-banked, Cat. No. A37785) GlycanTune C+ Total Feed, GlycanTune Feed Kit C+ • Enables seamless transition from transient to stable production without additional medium optimization • Use ExpiCHO™ Expression Medium (Cat. No. A29100) for transfection A37110-01 ExpiCHO Stable Production AGT Medium, granulated powder 10 L A37111-01 100 L A37111-02 450 L A37111-03 High-Intensity Perfusion CHO Medium (HIP CHO), AGT granulated powder 1 x 2 L DG44, CHO K1, GS CHO, and CHO-S cell lines designed for high-density At full medium concentration, designed to be used without additional feed • Designed to work with all types of suspension continuous perfusion processes • concentrations for both seed train and production work with cell lines that may be sensitive to more concentrated media • Supports high cell densities while reducing medium exchange rates—results from continuous perfusion of >100 million cells/mL at 1 VVD A42302-01 15 x 2 L A42302-02 100 x 2 L A42302-03 370 x 2 L A42302-03 CHO Cell Culture Medium, liquid and dry powder 1,000 mL DG44, CHO-K1, other CHO cell lines, and BHK-21 cells NA • A high-performance, complete formulation yielding cell growth and production of antibodies and recombinant proteins in suspension without the need for further supplementation • Contains Gibco™ Difco™ 220253 10 kg 670006 CD-1000 Medium, liquid and dry powder 1,000 mL CHO, NS0, and hybridoma cells Various hydrolysates or CD supplements • Designed as a platform medium suitable for multiple cell types • Ideally suited for further hydrolysate or chemically and perfusion processes for both research and manufacturing applications 215204 20 L BPC 215205 500 g 215206 10 kg 215207 Easy-Adapt™ CHO-K1 CD Medium, liquid 1,000 mL CHO-K1 and CHO NA • CHO-K1 cells need; rich in carbohydrates, amino acids, and vitamins 670112 20 L BPC 670114 CHO Media Panel 9 x 500 mL Diverse media panel designed to identify optimal formulation for various CHO cell lines CHO catalog feeds or CHO feed panel • Diverse library of formulations • Evaluation protocol • Technical evaluation support • Easy to customize • Scalable to rapid prototyping and GMP Order through Gibco Custom Media Services (gibcopanelteam@ ) ™ HT Supplement (Cat. No. 11067). Note: Express Services, go to Click on the product name to open the product details page. CHO Medium, dry powder 10 kg 670006 Order through Gibco Media by Design Services Contact Gibco Media by Design Services, go to thermofisher.com/mediabydesign, or contact your local FAS through your account manager. Advanced Granulation Technology Advanced Granulation Technology (AGT) media is a granular dry media format produced through a technologically advanced process which allows manufacturing of complete formulations in a variety of serum-free, protein-free and chemically-defined media in a dry format. Click here to learn more. 14 Product list for CHO cells Chemically defined feed supplements Peptones for CHO cell culture applications Tour our facilities virtually For nutrient replacement or glycan modulation in cell culture production of recombinant proteins, monoclonal antibodies, and vaccines; all are chemically defined, AOF, protein-free, and without phenol red, glutamine, hydrolysates, lipids, or growth factors, unless noted otherwise. Resurge™ CD1 Supplement, dry powder (Also see Resurge CD Pak) 100 g • A 5-member family of diverse nutritional feed supplements, without glucose and glutamine, developed using several CHO cell lines and various batch or fed-batch culture systems • feeding strategy • All 5 supplements should be concurrently evaluated your cell line and system Various basal media for CHO cell lines 670011 1 kg 670012 5 kg 670013 Resurge CD2 Supplement, dry powder (Also see Resurge CD Pak) 100 g 670015 1 kg 670016 5 kg 670017 Resurge CD3 Supplement, dry powder (Also see Resurge CD Pak) 100 g 670018 1 kg 670019 5 kg 670020 Resurge CD4 Supplement, dry powder (Also see Resurge CD Pak) 100 g 670021 1 kg 670022 5 kg 670023 Resurge CD5 Supplement, dry powder (Also see Resurge CD Pak) 100 g 670024 1 kg 670025 5 kg 670026 Resurge CD Pak 5 dry powder supplements 1. Resurge CD1 Supplement (Cat. No. 670011) 2. Resurge CD2 Supplement (Cat. No. 670015) 3. Resurge CD3 Supplement (Cat. No. 670018) 4. Resurge CD4 Supplement (Cat. No. 670021) 5. Resurge CD5 Supplement (Cat. No. 670024) 100 g of each 670030 CHO Feed Panel 5 x 100 mL • Diverse library of feeds designed to identify optimal formulation • Evaluation protocol • Technical evaluation support • Easy to customize • Scalable to rapid prototyping and GMP CHO catalog media or CHO media panel formulation Order through Gibco Custom Media Services (gibcopanelteam@ ) Note: , or contact your local FAS through your account manager. Gibco product and format Quantity Features Recommended Gibco basal medium Cat. No. ™ A+ Supplement Concentrated 3X, liquid 1,000 mL • Liquid is pH neutral at 3X concentrate • AGT format can be used from 1x to 3x by altering the ratio of powder to water • Total feed volumes generally range from 10–45% (1x) to 3–15% (3x) of the starting culture volume as further outlined in the user guide CD OptiCHO Medium A39374-01 10 L BPC A39374-02 Supplement, granulated powder 1 L A25023-04 10 L A25023-05 100 L A25023-01 GlycanTune™ A+ Total Feed, AGT granulated powder 1 L • Modulates glycan expression from G0F to desired existing feed replacement with the GlycanTune feed A29719-04 10 L A29719-05 100 L A29719-01 GlycanTune Feed Kit A+, AGT granulated powder 2 L (1 L each) • Supplement and GlycanTune A+ Total Feed A33159-01 ™ B+ Supplement Concentrated 3X, liquid 1,000 mL • Liquid is a pH-neutral 3X concentrate • AGT medium can be used from 1x to 3x by adding • Total feed volumes typically range from 10–45% (1x) to 3–15% (3x) of the starting culture volume, as further outlined in the user guide CD CHO Medium A39375-01 10 L BPC A39375-02 Supplement, granulated powder 1 L A25030-04 10 L A25030-05 100 L A25030-01 GlycanTune™ B+ Total Feed, AGT granulated powder 1 L • Modulates glycan expression from G0F to desired existing feed replacement with the GlycanTune feed A29720-04 10 L A29720-05 100 L A29720-01 GlycanTune Feed Kit B+, AGT granulated powder 2 L (1 L each) • Supplement and GlycanTune B+ Total Feed A33160-01 Gibco product and format Quantity Features Recommended Gibco basal medium Cat. No. Click on the product name to open the product details page. Contact Gibco Media by Design Services for details Contact Gibco Media by Design Services, go to thermofisher.com/mediabydesign, or contact your local FAS through your account manager. 15 Product list for CHO cells Peptones for CHO cell culture applications Tour our facilities virtually Peptone selection in CHO cell cultures For nutrient supplementation in mammalian, insect, and microbial cell culture production of recombinant proteins, monoclonal antibodies, and vaccine products. GibcoTM product and format Quantity Features Cat. No. Starter Pak No. 1 5 dehydrated powders*: 1. Difco™ Yeast Extract, ultra-filtered (UF) (Cat. No. 210929) 2. Bacto™ Yeast Extract, technical (Cat. No. 288620) 3. Bacto TC Yeastolate (Cat. No. 255772) 4. Difco TC Yeastolate, UF (Cat. No. 292804) 5. Difco Phytone Supplement, UF (Cat. No. 210931) 100 g of each • Starter sample package of five AOF Difco and Bacto peptones for use with mammalian and microbial cell cultures to enhance growth and production in batch or fed-batch processes • Evaluation of all five peptones in concurrent testing is recommended to determine which nutritional profiles are most beneficial to your culture 215366 * These are non-GMP samples of the same formulation as the catalog products listed; see footnote below. Starter Pak No. 2 6 dehydrated powders*: 1. Phytone™ Peptone (Cat. No. 211906) 2. Difco Soytone (Cat. No. 212488) 3. Bacto Yeast Extract (Cat. No. 212750) 4. Bacto Proteose Peptone No. 2 (Cat. No. 212120) 5. Bacto Proteose Peptone No. 3 (Cat. No. 211693) 6.Bacto Casamino Acids (Cat. No. 223050) 100 g of each • Starter sample package of three AOF and three animal-origin (AO) Difco and Bacto peptones for use with mammalian and microbial cell cultures to enhance growth and production in batch or fed-batch processes • Evaluation of all six peptones in concurrent testing is recommended to determine which nutritional profiles are most beneficial to your culture 215367 * These are non-GMP samples of the same formulation as the catalog products listed; see footnote below. Starter Pak No. 3 6 dehydrated powders*: 1. Bacto TC Yeastolate (Cat. No. 255772) 2. Phytone Peptone (Cat. No. 211906) 3. Difco Soytone (Cat. No. 212488) 4. Bacto Yeast Extract (Cat. No. 212750) 5. Bacto Malt Extract (Cat. No. 218630) 6. Yeast Extract (Cat. No. 211929) 100 g of each • Starter sample package of six AOF Difco and Bacto peptones for use with mammalian and microbial cell cultures to enhance growth and production in batch or fed-batch processes • Evaluation of all six peptones in concurrent testing is recommended to determine which nutritional profiles are most beneficial to your culture 215368 * These are non-GMP samples of the same formulation as the catalog products listed; see footnote below. Peptone Preview Pak 5 dehydrated powders*: 1. Wheat 100, UF (Cat. No. 215380) 2. Cotton Peptone 100, UF (Cat. No. 215381) 3. Cotton Peptone 200, UF (Cat. No. 215382) 4. Soy 100 (Cat. No. 215383) 5. Yeast 100 (Cat. No. 215384) 100 g of each • Features new wheat, cotton, soy. and yeast peptones which provide unique nutritional profiles compared to other cell culture supplements • Suitable for use with mammalian and microbial cell cultures to enhance growth and production in batch or fed-batch processes • Evaluation of all five peptones in concurrent testing is recommended to determine which nutritional profiles are most beneficial to your culture 215379 * These are non-GMP samples of the same formulation as the catalog products listed; see footnote below. Note: This chart provides information pertaining to Peptone Starter and Preview Paks for evaluation purposes. A complete listing of all catalog peptones supplements can be found in our Technical Guide to Peptones, Supplements, and Feeds at thermofisher.com/peptones GibcoTM product and format Quantity Features Recommended Gibco basal medium Cat. No. EfficientFeed™ C+ Supplement Concentrated 2X, liquid 1,000 mL • Liquid is pH neutral at 2X concentrate • AGT format can be used from 1x to 2x by alterin the ratio of powder to water • Total feed volumes generally range from 10–40 (1x) to 5–20% (2x) of the starting culture volume as further outlined in the user guide Dynamis Medium, ExpiCHO Stable Production Medium A39376-01 10 L BPC A39376-02 EfficientFeed C+ AGT, Supplement, granulated powder 1 L A25031-04 10 L A25031-05 100 L A25031-01 GlycanTune™ C+ Total Feed, AGT granulated powder 1 L • Modulates glycan expression from G0F to desired G1F and G2F profile based on shifting the timing of existing feed replacement with the GlycanTune feed A29721-04 10 L A29721-05 100 L A29721-01 GlycanTune Feed Kit C+, AGT granulated powder 2 L (1 L each) • Contains 1L each of EfficientFeed C+ AGT supplement and GlycanTune C+ Total Feed A33161-01 Click on the product name to open the product details page. Chemically defined feed supplements 16 Product list for CHO cells Peptones for CHO cell culture applications Tour our facilities virtually Chinese hamster ovary (CHO) Cell Line GibcoTM product and format Quantity Gibco optimized cell lines Gibco recommended feeds and supplements Features Cat. No. Freedom™ ExpiCHO-S kit - Kit contains ExpiCHO-S cells (cGMP-banked) and media EfficientFeed™ C+ Supplement or Efficient-Pro™ Feed 2 • Easy-to-use, beginning-to-end product, for the cloning and expression of recombinant proteins in CHO-derived suspension culture • Freedom ExpiCHO-S Kit includes components for transfection, expression, clone creation, and stable cell line selection, all in a conveniently packaged kit A46847 Chemically defined feed supplements GibcoTM product and format Quantity Features Recommended Gibco basal medium Cat. No. Efficient-Pro™ Feed 1, Liquid 1000 mL • Efficient-Pro Feed 1 for CHO-K1 and GS • Efficient-Pro Feed 2 for CHO-S, DG-44, and GS • AGT type: Ease-of-use associated with auto-pH and simplified dissolution • The Efficient-Pro system increases titer and specific productivity, improves protein quality and metabolite profiles, and is easier to use with less solubility issues than other CHO media and feed offerings Efficient-Pro Medium A5208801 Efficient-Pro™ AGT™ Feed 1, granulated powder 1 L A5209101 10 L A5209102 100 L A5209103 Efficient-Pro™ Feed 2, Liquid 1000 mL A5221404 Efficient-Pro™ AGT™ Medium, granulated powder 1 L A5221601 10 L A5221602 100 L A5221603 Additional products Chinese hamster ovary (CHO) media GibcoTM product and format Quantity Gibco optimized cell lines Gibco recommended feeds and supplements Features Cat. No. Efficient-Pro™ Medium, Liquid 1000 mL CHO-DG44, CHO-S, CHO-K1, GSCHO Efficient-Pro Feed 1, Efficient-Pro Feed 2 • AGT type: Ease-of-use with auto-pH and simplified dissolution • Sustain cell viability for longer bioreactor runs and maximized titer • Improve downstream outputs such as protein glycosylation, charge-variants, and aggregation • Speed up process development and streamline transfer to manufacturing scale • Increase flexibility with a range of format and packaging configurations • Decrease cell shock with simplified adaptation protocols A5322201 10 L A5322202 20 L A5322203 Efficient-Pro™ AGT™ Medium, granulated powder 1 L A5322301 10 L A5322302 100 L A5322303 500 L A5322304 Click on the product name to open the product details page. Efficient-Pro spotlight Learn more about our Gibco Efficient-Pro Medium and Feed System, designed to drive productivity in your batch or fed-batch CHO process. Watch the video Watch our webinar Read the application note Global network of R&D and manufacturing facilities 17 *R&D facilities: Grand Island (NY, USA), Baltimore (MD, USA), Singapore, and Shanghai (China) *Bioprocess Design Center: Singapore, Korea, and China North America • Grand Island, New York – Media, reagents, sera – Media & analytical services – Gibco rapid prototyping services – ISO 13485, 21 CFR 820 – MDSAP certified – FDA registered • Miami, Florida – Media, reagents – Animal-free facility – ISO 9001 • Detroit, Michigan – Peptones – ISO 13485 • Baltimore, Maryland – Media & analytical services – Gibco rapid prototyping services Europe • Paisley, Scotland – Media, reagents, sera – ISO 13485, 21 CFR 820 – FDA registered • Vilnius, Lithuania – Magnetic beads Asia-Pacific • New Zealand & Australia – Sera, animal proteins – ISO 13485, 21 CFR 820 – FDA registered • Shanghai & Suzhou, China – Media & analytical services (Shanghai) – Gibco rapid prototyping services (Suzhou) Media format of manufacturing facilities Media Format Grand Island, NY Paisley, Scotland Miami, FL Detroit, MI Advanced Granulation Tehnology (AGTTM) Animal Origin-Free Dry Powder Animal Origin Dry Powder Animal Origin Peptones Pin mill and FitzMillTM milling technology offered Learn more about our innovative Advanced Granulation Technology (AGT) media format and how it can benefit your process. Peptones for CHO cell culture applications Tour our facilities virtually Tour our facilities virtually For Research Use Only. Not for use in diagnostic procedures. © 2023 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Take a 360° tour of any one of our cell culture media manufacturing facilities today. Learn about our approach to site-to-site equivalency. Learn more at thermofisher.com/chomedia 18 Want to learn more about how we can support your scale-up journey? Whether you’re looking to reduce risk from supply chains or increase your capacity, you may need to consider an additional supplier. Discover more by clicking on the resources below. • Discover how to successfully qualify a reliable secondary supplier. • Streamline your proprietary media and scale up with Gibco. • Accelerate your cell culture media manufacturing from rapid prototyping through cGMP. Read the article Learn more in the brochure Discover more in the article Learn more about our global facilities Baltimore, MD [Click to take a tour] Miami, FL [Click to take a tour] Grand Island, NY [Click to take a tour] Could our CHO cell culture solutions meet your biomanufacturing needs? Contact us to learn more and talk to our team Paisley, Scotland [Click to take a tour] Contact us Peptones for CHO cell culture applications