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APP NOTE

Determination of KD of Aptamer Protein Interactions by Microfluidic Diffusional Sizing

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Fludic-LP-June2

The interactions of proteins with secondary molecules are of key importance across the life sciences.

While methods exist to determine KD experimentally, these often require a degree of expertise to collect reliable data and can have technique specific limitations.

This application note presents a new, simple and rapid technique to characterize protein interactions in vitro; microfluidic diffusional sizing (MDS).

Download this application note to learn a simple means to experimentally determine KD:

  • Without the need to alter molecules
  • No surface fixing
  • No complex preparation steps