How To Validate Your Antibodies
Antibodies are an invaluable tool in the laboratory. Whether imaging cells, checking for the presence of a specific protein or developing a diagnostic test, antibodies can hold the key to your needs. BUT, scientists beware! Failure to validate one’s experimental antibodies effectively prior to use can lead to non-reproducible results, false positives and negatives and many wasted hours, reagents and samples – particularly problematic for precious material.
Download this how-to guide to learn:
- The importance of context in optimization
- Why controls can make or break an assay
- How sample status may be confirmed in an antibody-independent manner
Find out how you could improve your immunoassays, including western blots, enzyme-linked immunosorbent assays, lateral flow assays and immunohistochemistry.