Fast, Simple Test for Colitis
News May 16, 2016
The technique involves testing serum, the clear liquid that can be separated from clotted blood, for the increased presence of mannose, a sugar that is a marker for colitis, using Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy. This technology is sensitive to vibrations in the chemical bonds of the serum sample’s molecules and requires minimal sample preparation, making it a rapid diagnostic alternative.
“We found that ATR-FTIR spectroscopy is an effective tool for detecting colitis in the serum of mice,” said Unil Perera, a Regents’ Professor of Physics and researcher in the Center for Nano-Optics at Georgia State. “This rapid, simple, cost-effective and minimally invasive technique could be further developed into a personalized diagnostic tool that would assess disease status based on an individual’s molecular composition and allow for personalized diagnosis and drug management. Perhaps this technology could be integrated into a portable device, such as the glucometer used by patients with diabetes.”
Inflammatory bowel diseases, which include ulcerative colitis and Crohn’s disease, involve chronic inflammation of all or part of the digestive tract and can lead to life-threatening complications such as colorectal cancer. Assessing this inflammation remains a challenge, and clinical diagnosis is now achieved by colonoscopy, which uses an endoscope or flexible tube with a light and camera attached to examine the digestive tract. This technique is not ideal for an annual checkup or monitoring disease activity regularly because it’s expensive, invasive and requires sedation.
As a result, “there is a need for a simple, inexpensive and low-risk diagnostic tool for inflammatory bowel diseases,” said Didier Merlin, professor in the Institute of Biomedical Sciences at Georgia State and a researcher at the Atlanta Veterans Affairs Medical Center. “Infrared spectroscopy has greatly enhanced clinical medicine in the last two decades and shows promise as a solution.”
In the study, mice in two categories were tested for colitis using ATR-FTIR spectroscopy. The first group was mice with targeted deletion of the interleukin 10 (IL10) gene, known as interleukin 10 knockout (IL10-/-) mice, which develop colitis through T helper immune cells. Disease in IL10-/- mice closely resembles the physiological, histological and biochemical features of chronic colitis in humans. In the second group of mice, colitis was induced by administering Dextran Sodium Sulphate. Colitis in these mice is similar to ulcerative colitis in humans. In both groups, feces and blood samples were collected and tested. Mice in control groups were also tested.
The researchers found ATR-FTIR spectroscopy is an effective tool for detecting colitis in mice serum, showing a significant increase in the levels of mannose, an indicator of colitis. The Georgia State University Research Foundation has filed a provisional patent for the screening technique.
Record Concentration of Microplastic in Arctic Sea IceNews
Experts have recently found higher amounts of microplastic in arctic sea ice than ever before, the majority of which were microscopically small. However, the different types of plastic showed a unique footprint in the ice allowing the researchers to trace them back to possible sources.READ MORE
Tunable Infrared Filter Aids Soldiers In the FieldNews
Soldiers need to see through airborne obscurants and detect the presence of toxins or other chemicals in the field. To identify those chemicals, they use infrared sensors and spectroscopy. Researchers have now developed a tunable infrared filter made from graphene, removing the need to change goggles for different applications.READ MORE
Smart Phone Developed for Quicker Infection TestingNews
Washington State University researchers have developed a low-cost, portable laboratory on a phone that works nearly as well as clinical laboratories to detect common viral and bacterial infections.READ MORE
Comments | 0 ADD COMMENT
15th Symposium on the Practical Applications of Mass Spectrometry in the Biotechnology Industry
Sep 09 - Sep 12, 2018
CE in the Biotechnology & Pharmaceutical Industries: 20th Symposium on the Practical Applications for the Analysis of Proteins, Nucleotides & Small Molecules
Sep 09 - Sep 12, 2018