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Mass Spectrometry Shows Potential for Rapid SARS-CoV-2 Detection
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Mass Spectrometry Shows Potential for Rapid SARS-CoV-2 Detection

Mass Spectrometry Shows Potential for Rapid SARS-CoV-2 Detection
News

Mass Spectrometry Shows Potential for Rapid SARS-CoV-2 Detection

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Rapid but yet sensitive, specific and high-throughput detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in clinical samples is key to diagnose infected people and to better control the spread of the virus. Alternative methodologies to PCR and immunodiagnostic that would not require specific reagents are worth to investigate not only for fighting the COVID-19 pandemic, but also to detect other emergent pathogenic threats.

In the present study, scientists from SPI (Marcoule) propose the use of tandem mass spectrometry to detect SARS-CoV-2 marker peptides in nasopharyngeal swabs. They documented that the signal from the microbiota present in such samples is low and can be overlooked when interpreting shotgun proteomic data acquired on a restricted window of the peptidome landscape.

In this proof of concept study, simili nasopharyngeal swabs spiked with different quantities of purified SARS-CoV-2 viral material were used to develop a nanoLC-MS/MS acquisition method, which was then successfully applied on COVID-19 clinical samples. Researchers argue that two peptides from the nucleocapsid protein are of utmost interest as their signal is intense and their elution can be obtained within a 3 min window in the tested conditions.

These results pave the way for the development of time-efficient viral diagnostic tests based on mass spectrometry.

Reference
D. Gouveia, G. Miotello, F. Gallais, JC. Gaillard, S. Debroas, L. Bellanger, JP. Lavigne, A. Sotto, L. Grenga, O. Pible, J. Armengaud. Proteotyping SARS-CoV-2 virus from nasopharyngeal swabs: a proof-of-concept focused on a 3 min mass spectrometry window. J. Proteome Res., 2020, http://dx.doi.org/10.1021/acs.jproteome.0c00535

This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source.

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