Researchers Uncover new Genetic Clue to Leukemia’s Resistance to Treatment
News Mar 22, 2010
The gene that causes a form of leukemia also appears to spawn mutations in itself, leading to drug-resistant disease, according to City of Hope researchers. The finding provides a new tool for understanding how resistant disease develops and could lead to methods to overcome it.
Reporting in the Feb. 12 issue of The Journal of Biological Chemistry, WenYong Chen, Ph.D., assistant professor of biology, and a team of researchers sought to understand how mutations in a gene called BCR-ABL make chronic myelogenous leukemia, or CML, resistant to drugs such as imatinib.
WenYong Chen (Photo by Darrin S. Joy)
BCR-ABL is an abnormal gene formed when genes from two different chromosomes mistakenly join together in a bone marrow cell. The abnormal gene makes a new “fusion protein” that spins white blood cells out of control and causes CML.
Imatinib, also known as Gleevec, revolutionized CML treatment by allowing patients to control their disease with a daily pill. The drug disables the BCR-ABL protein, which the BCR-ABL gene makes.
Over time, though, the BCR-ABL gene changes and mutates, which alters the protein so that imatinib no longer recognizes it. That leads to disease that resists imatinib.
City of Hope researchers wanted to follow cells’ path to drug resistance. They grew CML cells in the presence of imatinib in the lab and carefully tracked cells’ growth pattern as they became drug resistant and flourished.
The pattern showed that the cells suddenly developed new mutations in the BCR-ABL gene. Surprisingly, though, when researchers completely turned the BCR-ABL gene off before adding imatinib to the cells, the BCR-ABL gene did not mutate. They found the BCR-ABL gene was more prone to mutate when it was on its native chromosome than on other chromosomes.
That means that BCR-ABL’s gene activity actually causes it to mutate.
“We knew from previous research that BCR-ABL was involved with its own mutation, but we didn’t realize its gene activity was required,” said Chen. The finding suggests that BCR-ABL activity is the driving force that makes CML cells genetically unstable and prone to mutation.
Further studies by the team will aim to uncover more details about the underlying processes leading to CML drug resistance with the aim of boosting treatment with Gleevec and similar drugs.
A team led by Johns Hopkins Medicine researchers says it has identified two protein biomarkers in urine that may one day be used to better diagnose acute interstitial nephritis, an underdiagnosed but treatable kidney disorder that impairs renal function in the short term and can lead to chronic kidney disease, permanent damage or renal failure if left unchecked.READ MORE
Some MRSA infections could be tackled using widely-available antibiotics, suggests new research. A team of scientists used genome sequencing technology to identify which genes make MRSA susceptible to a previously defined combination of drugs. They identified a number of mutations centered around a protein known as a penicillin-binding protein 2a or PBP2a.