Profiling Personal Air Pollutant Exposures Using a Wearable Non-Selective Passive Wristband
Characterizing cumulative exposure to air pollutant mixtures is a critical step in understanding disease development. The Fresh Air wristband was tested as a personal exposure assessment tool with promising results.
P450 Induction in Cryopreserved Hepatocytes from PXR and CAR Nuclear Receptor Knock-out Rats
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.
Mass Spectrometry: From Imaging to Metabolic Networks
We show that network analysis of co-localized ions from mass spectrometry imaging data provides a detailed chemo-spatial insight into the metabolic heterogeneity of tumors. Furthermore, module preservation analysis between colorectal cancer patients with and without metastatic recurrence suggests hypotheses on the nature of the different local metabolic pathways.
A new method for generating arrayed RNAi screening tools for any organism
RNA interference (RNAi) using small interfering RNAs (siRNAs) is an important technology for down-regulation of gene expression and a powerful tool to study cellular processes and pathways. Previously, large collections of siRNAs were available only for traditional experimental model systems, such as human and mouse, and predominantly provided as chemically synthesized libraries.
Idiopathic Pulmonary Fibrosis: The Importance of Multiple-site Sampling
This research project attempts to highlight the importance of sampling from multiple sites of the lungs of patients with Idiopathic Pulmonary Fibrosis. Examining the histopathology of the human lung samples with IPF shows varying levels of fibrosis in different sites of the lung. To ensure an accurate representation of a patient's lungs, it is important to obtain samples from multiple sites on the lobes of the lungs.
Hepatocellular Carcinoma with Small Cell Histology: A Rare Mimic of Metastatic Tumors Involving the Liver
Two cases of hepatocellular carcinoma (HCC) with small cell histology, a rare morphologic variant of HCC, are presented.
Extracorporeal shockwave therapy accelerates motor axon regeneration despite a phenotypically mismatched environment
A femoral nerve defect model was adapted for the evaluation of proregenerative effects of extracorporeal shockwave therapy (ESWT). Functional evaluation, histology and qRT-PCR data show differences between sensory and motor-derived nerve transplants and a pro-regenerative effect of ESWT. These data provide evidence for the clinical application of ESWT after autologous nerve transplantation as a novel non-invasive method.
Severe Combined Immunodeficiency (SCID) caused by thymic aplasia due to a mutation in TBX1
We report one of the first cases of TBX1 haploinsufficiency causing complete thymic aplasia and SCID.
Fast arsenic speciation analysis of wines and rice with LC-ICP-QQQ
This method was designed in response to recent and proposed food standards, both international and national, that limit inorganic arsenic rather than total, organic, or individual arsenic species such as arsenite (AsIII) and arsenate (AsV). Analysis time is 10x faster than the current FDA regulatory method, increasing sample throughput, avoided spectral interferences and dramatically increased sensitivity. Validation data from two laboratories demonstrate the method’s accuracy and reproducibility of both wine and rice matrices in a single analytical batch.
Proteomics and Substrate Based MS Imaging of Xenobiotic Metabolising Enzymes in Ex Vivo Human Skin and a Human Living Skin Equivalent Model
Untargeted proteomics analysis showed that human skin and a commercially available living skin equivalent model exhibit a similar distribution of xenobiotic metabolising enzymes. A new technique, substrate based mass spectrometry imaging (SB-MSI) was developed during this study.