A new method for generating arrayed RNAi screening tools for any organism
Poster May 08, 2018
Jesse Stombaugh, Megan Basila, Amanda Haas, Annaleen Vermeulen, Melissa L. Kelley, Anja van Brabant Smith
For studies in alternate species, siRNAs needed to be custom synthesized at a relatively high cost and very long manufacturing time. To improve the accessibility of these large screening libraries to researchers working in non-human or mouse model systems, we have recently developed a new cost-effective enzymatic manufacturing method to generate large collections of siRNAs, called Dharmacon™ Zoonome™ siRNAs (z-siRNAs). Like other well-established siRNA product lines, they are designed using the proprietary SMARTselection design algorithm for potent and specific gene expression knockdown, but are manufactured on-demand using a novel enzymatic method. Now available are customized arrayed collections of pools of four z-siRNAs to each gene target for screening hundreds or thousands of genes for any organism with an annotated genome. Here we show successful gene knockdown in Cricetulus griseus (Chinese hamster) and Bos taurus (bovine) cell lines using Zoonome siRNAs. With this new availability of large screening libraries, the power of unbiased functional screening in nearly any species can accelerate findings important to animal health, food safety, and infectious disease.
Characterization of a Type 2 diabetes-associated islet-specific enhancer cluster in STARD10 by genome editing of EndoC-βH1 cellsPoster
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE
P450 Induction in Cryopreserved Hepatocytes from PXR and CAR Nuclear Receptor Knock-out RatsPoster
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.