Applications of EFC-based Protein-Protein Interaction Assays for Gi, Gs, and Gq-Coupled GPCR Screening
Poster Dec 13, 2007
GPCR targets can be addresssed with a variety of technologies and they are increasingly being evaluated with multiple assay approaches. DiscoveRx has validated over 60 GPCR targets (Gi, Gs, Gq, Type A & Type B) in a novel generic cell-based assay format utilizing protein-protein interaction batween the GPCR C-terminus and b-arrestin2. Data outlining benefits provided for primary & secondary screening is presented.
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE