Idiopathic Pulmonary Fibrosis: The Importance of Multiple-site Sampling
METHODS: This research project involving the procurement and use of lung samples from patients with IPF was reviewed and approved by IACUC of University of South Florida (Pro00020884). Lung samples were collected from a patient who underwent bilateral lung transplantation at Tampa General Hospital. 3 lung blocks, 3-cm-sized for each, were excised from each of right and left lower lobe. Paraffin-embedded sections prepared from each part of lung was stained with H&E. Histopathological evaluation was performed under light microscope.
RESULTS: Samples collected from different areas of lung tissue of one patient with IPF showed varied histopathological apperances. Fibroblastic foci are are seen in most of the areas. Adjacence of vascular remodeling and bronchiolar epithelial hypercellularity are noted in a patchy manner.
CONCLUSION: Lung tissue shows varying levels of progression of fibrosis in different sections of the tissue in IPF. This highlights the importance of sampling from multiple sites on the lobes of the lung to ensure an accurate representation of a patient’s lungs.