Idiopathic Pulmonary Fibrosis: The Importance of Multiple-site Sampling
Poster Apr 18, 2018
Sudarshan Krishnamurthy, Jutaro Fukumoto, Sahebgowda Patil, Irene John, Helena Hernandez-Cuervo, Matthew Alleyn, Mason Breitzig, Lakshmi Galam, Ramani Soundararajan, Kapilkumar N. Patel, Richard F Lockey, Narasaiah Kolliputi
RATIONALE: Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive lung disease with no effective treatments available. Histology of IPF manifests in several different ways including, but not limited to, fibroblastic foci, bronchiolar epithelial hypercellularity and narrowing of vasculatures and airspaces. The histopathological evaluation of IPF lungs in basic research is often conducted based on samples collected from limited area due to the difficulty of obtaining IPF lung samples. Therefore, using multiple lung samples from different sites of the lung of patient who undergoes transplantation is very important and may give us valuable information.
METHODS: This research project involving the procurement and use of lung samples from patients with IPF was reviewed and approved by IACUC of University of South Florida (Pro00020884). Lung samples were collected from a patient who underwent bilateral lung transplantation at Tampa General Hospital. 3 lung blocks, 3-cm-sized for each, were excised from each of right and left lower lobe. Paraffin-embedded sections prepared from each part of lung was stained with H&E. Histopathological evaluation was performed under light microscope.
RESULTS: Samples collected from different areas of lung tissue of one patient with IPF showed varied histopathological apperances. Fibroblastic foci are are seen in most of the areas. Adjacence of vascular remodeling and bronchiolar epithelial hypercellularity are noted in a patchy manner.
CONCLUSION: Lung tissue shows varying levels of progression of fibrosis in different sections of the tissue in IPF. This highlights the importance of sampling from multiple sites on the lobes of the lung to ensure an accurate representation of a patient’s lungs.
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Comments | 2 ADD COMMENT
Fred Gilberts | May 21, 2018
A CT scan in August of 2009 verified that I had Idiopathic Pulmonary Fibrosis. My first symptoms were cough and shortness of breath. I was on prednisone and inhalers. My blood oxygen level was 50 and i was extremely short of breath, i was barely able to breath. I went through cardio pulmonary rehab, It helped but not too long before all the severe symptoms returned. December last year, a family friend told us about Rich Herbs Foundation and their successful lungs disease treatments, we visited their website ww w. richherbsfoundation. c om and ordered their IPF herbal treatment, i am happy to report this treatment effectively reversed my Idiopathic pulmonary fibrosis and symptoms. I am back on my feet, i walk daily now and has made me able to walk my two dogs again without shortness of breath or sudden loss of energy. My activity level is up again.
Sorice Madina | May 09, 2018
I was diagnosed with Idiopathic pulmonary fibrosis. After a two-week stay in the hospital, I was home with oxygen. I was still coughing badly, breathless and my chest pain was ongoing. I had a heart attack six months ago. I'm 69, female, and no longer working. I lost 36 pounds in 1 month as my coughing causes me to vomit non-stop! I was so tired. My blood pressure has always been high; it averages at 193/88, I was on minimum daily dosage prednisone (7.5 mg), azathioprine and N-Acetyl. They put me on 6 liters of oxygen and steroids. The pulmonary doctor told me that I was going to die and said there was nothing he could do for me,I started on Natural Herbal Gardens IPF Herbal formula treatment in June 2017, i read alot of positive reviews on their success rate treating IPF disease through their Herbal formula and i immediately started on the treatment. Just 7 weeks into the Herbal formula treatment I had great improvements with my breath and my chest pain was no more. I am unbelievably back on my feet again, this is a breakthrough for all Pulmonary Fibrosis sufferers, visit Natural Herbal Gardens official website www . naturalherbalgardens. com i turned 69 today. i will keep sharing awareness