Onchocerca Diagnostic Testing of Polymorphisms; Effects of Antigenic Mutations on Detection of Antibodies by OV-16 ELISA
Poster Feb 14, 2017
Kristi Miley, Hassan Hassan, Canhui Liu and Thomas R. Unnasch
Onchocerca is a filarial parasite transmitted to humans by the female blackfly of the Simulium genus that is known to cause “river blindness” among humans in regions of Africa and the Americas. ELISA (Enzyme-linked Immunosorbent Assay) serological testing for the presence of antibodies to a diagnostic antigen (Ov16) is a diagnostic tool in current use for verifying suppression of transmission of the parasite. Recently a humanized monoclonal antibody has been developed to use as a positive control in the Ov16 ELISA. This was done as it is becoming harder to obtain positive samples for use as pooled sera for a source of positive controls as elimination grows closer. Recently we have discovered that naturally occurring polymorphisms in the Ov16 antigen affect the binding of this monoclonal antibody. Here, we have identified the particular polymorphism responsible for the loss of monoclonal antibody reactivity and investigated the potential effect these polymorphisms may have on the sensitivity of the Ov16 ELISA for the detection of parasite exposure.
Complete alignment identification of CRISPR-Cas9 genomic off-targets using Edit-R CRISPR specificity tool and a comprehensive analysis of positional mismatch tolerancePoster
A web tool that performs complete crRNA specificity checking is introduced. In addition, we evaluated positional off-targeting of the CRISPR system.READ MORE
MicroRNAs as Bile-Based Biomarkers in Pancreatic Cancer and Biliary Tract CancersPoster
In this study, the role of miRNAs has been assessed in a large cohort of pancreatic cancer.READ MORE
Point of Care Ultrasound in Risk Stratification of Acute Pulmonary EmbolismPoster
We present a case of pulmonary embolism in a young man who
benefitted from point of care ultrasound. His management was
changed significantly with the results despite initial risk stratification
suggesting ambulatory management.
Comments | 0 ADD COMMENT
Theme: Gathering the Newborn Advances in Cardiology
Mar 19 - Mar 20, 2018