New England Biolabs® Launches NEBNext Direct® Custom Ready Panels for Efficient Targeted Re-sequencing
NEBNext Direct
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New England Biolabs (NEB®) announced the launch of the NEBNext Direct Custom Ready Panels. The new panels — coupled with the proprietary NEBNext Direct target enrichment technology — enable the rapid development and deployment of a customized target enrichment panel by allowing users to select from an extensive library of genes to produce sequencing data with high specificity and coverage uniformity.
“Targeted next generation sequencing panels for genomic profiling are generally plagued by design difficulty and high cost due to the wide variation in interrogated genes that are specific to a given study,” said Andrew Barry, Product Marketing Manager, Target Enrichment at NEB.
“As a result, we developed a library of pre-synthesized ‘baits’ that are optimized and specific to the full exonic content of ~850 genes associated with a range of human diseases, which can be readily combined into customized panels,” said Barry. “Not only does this give researchers more freedom in panel design, but also it allows them to simply and quickly do more meaningful work with clinically relevant samples.”
According to Dr. Guang Peng, M.D., Ph.D. of MD Anderson Cancer Center, "NEBNext Direct Custom Ready Panels have allowed my research to focus on the specific genes we need to explore. In addition to the convenience of easily selecting genes for focused panels, NEBNext Direct enrichment has provided the necessary reliability and depth of coverage to enable robust somatic variant calling."
NEBNext Direct Custom Ready Panels employ the NEBNext Direct target enrichment technology, which balances the speed and precision of multiplexed PCR-based approaches with the content scalability of hybridization-based methods. The approach rapidly hybridizes both strands of genomic DNA with biotinylated probes prior to streptavidin bead capture, enzymatic removal of off-target sequences, and conversion of captured molecules into sequence-ready libraries. A unique molecular identifier tags each individual molecule prior to the final PCR amplification to enable identification of PCR duplicates, and barcodes are added to each library during PCR for high-throughput pooling.
“Targeted next generation sequencing panels for genomic profiling are generally plagued by design difficulty and high cost due to the wide variation in interrogated genes that are specific to a given study,” said Andrew Barry, Product Marketing Manager, Target Enrichment at NEB.
“As a result, we developed a library of pre-synthesized ‘baits’ that are optimized and specific to the full exonic content of ~850 genes associated with a range of human diseases, which can be readily combined into customized panels,” said Barry. “Not only does this give researchers more freedom in panel design, but also it allows them to simply and quickly do more meaningful work with clinically relevant samples.”
According to Dr. Guang Peng, M.D., Ph.D. of MD Anderson Cancer Center, "NEBNext Direct Custom Ready Panels have allowed my research to focus on the specific genes we need to explore. In addition to the convenience of easily selecting genes for focused panels, NEBNext Direct enrichment has provided the necessary reliability and depth of coverage to enable robust somatic variant calling."
NEBNext Direct Custom Ready Panels employ the NEBNext Direct target enrichment technology, which balances the speed and precision of multiplexed PCR-based approaches with the content scalability of hybridization-based methods. The approach rapidly hybridizes both strands of genomic DNA with biotinylated probes prior to streptavidin bead capture, enzymatic removal of off-target sequences, and conversion of captured molecules into sequence-ready libraries. A unique molecular identifier tags each individual molecule prior to the final PCR amplification to enable identification of PCR duplicates, and barcodes are added to each library during PCR for high-throughput pooling.
