We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
TRAP Enzyme Histochemistry for Osteoclast Detection on Bone Sections now Established with TissueSurgeon
Product News

TRAP Enzyme Histochemistry for Osteoclast Detection on Bone Sections now Established with TissueSurgeon

TRAP Enzyme Histochemistry for Osteoclast Detection on Bone Sections now Established with TissueSurgeon
Product News

TRAP Enzyme Histochemistry for Osteoclast Detection on Bone Sections now Established with TissueSurgeon


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "TRAP Enzyme Histochemistry for Osteoclast Detection on Bone Sections now Established with TissueSurgeon"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

The detection of osteoclasts in bone section is not easy, as the cells do not appear in large numbers. They are multinucleated (up to 10 nuclei) and can be found on the bone surface in resorption bays, also called Howships lacunae. A very established way of osteoclast detection is TRAP staining (Tartrate Resistant Acid Phosphatase) which actually detects acid phosphatase enzyme vesicles inside the ostecoclasts. This method is used on either decalcified paraffin sections or ground sections. 

csm_rat_femur_9accc40902.jpg

For the first time TRAP staining method was proofed on non-decalcified thin sections performed with the Lasermicrotome TissueSurgeon at 10µm thickness. In comparison to decalcified paraffin sections, also hard tissue information is still available for analysis; compared to ground sections, laser sections are much thinner and therefore accessibility to microscopy is improved. In ground sections osteoclasts may be covered by other cell layer.

Preparation of thin sections is much faster and easier to perform with the laser compared to ground sectioning or paraffin sectioning. The establishment of TRAP enzyme histochemistry on thin sections cut with the lasermicotome TissueSurgeon demonstrates that the laser cutting does not destroy the enzyme function and therefore is suitable for enzyme histochemical analysis.

Advertisement