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Cell Culture – Multimedia

LacZ Reporter Fusion Assay for Rapid and Easy Identification of Highly Efficient siRNA and its Delivery by Lentivirus into Suspension Cell Lines content piece image
Poster

LacZ Reporter Fusion Assay for Rapid and Easy Identification of Highly Efficient siRNA and its Delivery by Lentivirus into Suspension Cell Lines

The LacZ reporter fusion assay is an excellent method to precisely quantify knockdown efficiency of siRNA-sequences and can be corroborated by Western blot analysis. Efficient production of Lentiviruses and high transduction efficiency (up to 98%) were achieved on lymphoid B- and T-cells as demonstrated by FACS analysis and GFP expression. Successful knockdown effect with specific shRNA was observed in suspension cells three days after lentiviral infection.
Determination of Interferon-Pathway–Related Gene Induction during RNAi  content piece image
Poster

Determination of Interferon-Pathway–Related Gene Induction during RNAi

Experimental approaches using short interfering RNA (siRNA) molecules to specifically silence gene expression have become more widely used in recent years. As for all techniques in molecular and cellular biology, the importance of protocol optimization and the use of appropriate controls cannot be overestimated.
Development of High-Throughput PAMPA as an In Vitro Model of Passive Transcellular Permeation content piece image
Poster

Development of High-Throughput PAMPA as an In Vitro Model of Passive Transcellular Permeation

The absorption of orally administered compounds is largely determined by their ability to cross the gastrointestinal tract. Cell culture models can be intensive and limited to a narrow pH range. Assays using artificial membranes, such as PAMPA (parallel artificial membrane permeation assay) can be used as an alternative approach to assess in vitro transcellular passive permeation.
Monitoring Translocation Activity of Transcription Factors and Nuclear Hormone Receptors Using EFC content piece image
Poster

Monitoring Translocation Activity of Transcription Factors and Nuclear Hormone Receptors Using EFC

Enzyme Fragment Complementation can provide data for analysis of protein translocation events in whole cells, with the added benefits of increased throughput, simplified luminescence detection, no antibody requirements and decreased assay costs.
From Medicinal Plants to Bioactive Drugs content piece image
Poster

From Medicinal Plants to Bioactive Drugs

The aim of our study was to analyze the antiproliferative activity effects on human tumor cell lines and the differentiating activity on human erythroleukemic K562 cells of different extracts derived from medicinal plants of Bangladesh and Lebanon.
High Throughput Automated High Content Screening using a BioCube™ System and Microplate Cytometry content piece image
Poster

High Throughput Automated High Content Screening using a BioCube™ System and Microplate Cytometry

Here, we model the integration of the Protedyne BioCube System and the Acumen Explorer for practical high-throughput, high content screening of protein kinases.
GPVI-RBL-2H3-NFAT-Re Luciferase Cell Line: a New Cell Based System for Studying Collagen Receptor Activation content piece image
Poster

GPVI-RBL-2H3-NFAT-Re Luciferase Cell Line: a New Cell Based System for Studying Collagen Receptor Activation

This study describes the generation of a cell based assay which allows the functional characterization of the human GPVI receptor, by the use ofluminescence.
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