Liquid Biopsies – Multimedia

This article highlights some of the wide range of applications which can be achieved using mass spectrometry.

Our objective is to validate diagnostic services using Oxford Nanopore’s Minion in the first instance and to evaluate the cost and performance compared to existing sequencing technology in areas such as tumour DNA sequencing (and circulating tumour DNA), virology, microbiology, genetics and HLA-typing.

We developed a magnetic bead-based cfDNA extraction kit and integrated it into the automated qPCR- and NGS-based IVD workflows. In this study we are compering this magnetic beads-based cfDNA extraction system with a column-based method.

Liquid biopsy provides a circulating biomarker not only for treatment response but decision making of a sequential strategy.

Seth Crosby, Washington University School of Medicine, discusses both bench and informatic methods used to increase specificity in the apprehension of rare cfDNA alleles.

Jeff Cummings presents a worked example of circulating tumour cell enumeration as a prognostic/predictive biomarker in cancer research.

Paramagnetic Dynabeads® magnetic beads provide a versatile, automation friendly tool for fast sensitive and high throughput isolation that can be tailored to isolate specific circulating biomarkers such as cells, exosomes and nucleic acids.

In patients with NSCLC, Electric Field–Induced Release and Measurement (EFIRM) can detect EGFR mutations directly in saliva. This enables clinicians to adjust their therapeutic strategies in a timely fashion, consequently improving the clinical outcome of EGFR-targeted therapy.

A novel amplicon approach allowing for hundreds of amplicons to be multiplexed in a single tube with a two workflow from sample to sequencer.

We show non-responders to treatment among breast cancer patients can be identified by expression profiling of circulating tumor cells