We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

Improvements in Molecular Diagnosis of Pathogens and Diseases

Video   Apr 28, 2014

 

About the Speaker


Prof. Panagiotis Karanis obtained his PhD in Parasitology from Bonn University.  His worldwide research activities focused in the control of waterborne and vectorborne parasitic diseases including the development of diagnostic assays useful for basic and clinical platforms in the field of biomedicine. He was the main speaker for the Noble-Days-Lecture during the Nobel-Days-Festivities at the Örebro University in Sweden in December 10th, 2012, focused on Malaria vaccine development. AbstractLoop-mediated Isothermal Amplification (LAMP) belongs to a range of main current and new diagnostic techniques for early-stage detection of pathogens such as viruses, bacteria, fungi, protozoa, worms, amongst other. In addition, molecular recognition of cancer, genetic disorders and species determination or sex determination of organisms has also achieved by LAMP. The LAMP reaction was found to be superior over the Polymerase Chain Reaction (PCR) in most cases with respect to detection limit, sensitivity, specificity and usefulness. The superior capability of LAMP relies on the application of primer sets consisting of four to six designated primers that recognize certain regions of the target nucleic acid sequence and on the incorporation of Bst DNA polymerase capable of splicing DNA strands. We can expect that research on and application of the LAMP method will take a course that is similar to that of the PCR method during the last decades, which means improvement of its already high sensitivity and specificity, improvement of its multiplexing capability, extension of its application fields to both highly specialized detection tasks and fields of economic interest and further cost reduction.

 
 
 
 

Recommended Videos

What is Leukemia?

Video

Stem cells found in the bone marrow are crucial for our health because they are needed to become new blood cells that sustain and protect our bodies. But when the transformation goes wrong, harmful mutations can cause the cells to start replicating without control -- a type of cancer known as leukemia.

WATCH NOW

Genetic Causes of Myeloma

Video

Myeloma occurs as a result of changes in the genetic material within plasma cells. This animation explains what happens at the genetic level which causes myeloma to develop.

WATCH NOW

Cancer Cell Biology: Mutated KRAS & Reciprocal Signaling

Video

Across a wide variety of cancer types, a protein called KRAS can get hyperactivated and transmit an overload of unwanted growth signals to the cells, causing them to divide and form a tumor.

WATCH NOW

 

Like what you just watched? You can find similar content on the communities below.

Cancer Research Genomics Research

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE