Maximizing Insights From a Single Sample With dPCR Multiplexing

Multiplex PCR is a powerful technique that enables the detection of more than one target in a single reaction. This method maximizes the gain of biological information even with limited sample amounts, budgets and time. However, in typical multiplexing, the number or targets is limited by the number of available detection channels for the dyes used to label the target-specific probes. As a result, most qPCR and dPCR systems detect no more than six targets in parallel. The QIAcuity Digital PCR System has overcome this limitation, enabling clear discrimination of up to 12 targets in a single reaction. This high-level multiplexing is made possible by specialized chemistry and advanced software with improved cross talk compensation, which corrects for signal overlap between targets. In this webinar, Dr. Gerald Schock, Director Product Management dPCR at QIAGEN, will explain how the combination of sophisticated software and innovative chemistry enables the analysis of up to 12-plex reactions. He will also present examples of 6-plex, 8-plex and 12-plex experiments for copy number variation (CNV) analysis and pathogen detection. A live Q&A will help you integrate multiplexing in your lab to enhance productivity and extract more information from your experiments. Multiplex PCR is a powerful technique that enables the detection of more than one target in a single reaction. This method maximizes the gain of biological information even with limited sample amounts, budgets and time. However, in typical multiplexing, most qPCR and dPCR systems detect no more than six targets in parallel. In this webinar, our expert speaker will discuss how the combination of sophisticated software and innovative chemistry enables the analysis of up to 12-plex reactions. He will also present examples of 6-plex, 8-plex and 12-plex experiments for copy number variation (CNV) analysis and pathogen detection. 1. Learn how to integrate multiplexing in your lab to enhance productivity and extract more information from your experiments 2. Hear how to overcome challenges in dPCR multiplexing 3. Gain insights into the applications of highly-multiplexed dPCR, including in pathogen detection and CNV analysis Gerald Schock, PhD Director of Product Management, dPCR Choose an item. Other: QIAGEN Dr. Gerald Schock studied Biology and Biochemistry at the University of Mainz, Germany and at the University College of Wales, Aberystwyth, UK. He joined QIAGEN after receiving his Ph.D. in 1996 and has held positions in Sales, Regional Marketing, and Global Marketing, where he built up the epigenetics portfolio, including Pyrosequencing instruments, before assuming responsibility for the Life Science detection portfolio and transitioning into digital PCR.