Measuring Metabolic Flux with Cellular and Molecular Resolution: Combining Seahorse XF and LC/MS Technologies for Comprehensive Cellular Analyses

Alterations in metabolic flux have been linked to a wide variety of cell processes, from cancer development to T cell activation during immunotherapy or infection. This talk will cover two complementary technologies for measuring metabolic flux: Seahorse XF analyzers and liquid chromatography-mass spectrometry (LC/MS) qualitative flux analysis. Both technologies provide important insights into the rates of metabolic reactions within cells, with Seahorse XF measurements providing cellular resolution, and LC/MS qualitative flux measurements providing molecular resolution.

Key learning objectives: 

• Gain familiarity with metabolic rate measurements accessible to users of Seahorse XF and LC/MS technologies.
• Understand biological insights that can be obtained through the combination of Seahorse XF and LC/MS measurements.

For Research Use Only. Not for use in diagnostic procedures.