Latest App Notes & Case Studies
App Note / Case Study
Automated sample preparation for the determination of testosterone in serum by LC-MSMS
For accurate, reproducible quantification of testosterone, a growing number of laboratories are shifting from immunoassay to liquid chromatography coupled with tandem mass spectrometry.
This application note describes the preparation of serum samples for subsequent LC-MS analysis of testosterone, focusing on a streamlined workflow suitable for automation.
App Note / Case Study
Creating dose-response curves for cell-based and biochemical assays with the HP D300 Digital Dispenser
Small molecule drug discovery relies on the evaluation of one compound against another compound. Measurement of the activity of the small molecule against a target must be standardized by determining the IC50 or EC50 value using does response curve. The classical method of generating a dose-response curve is via serial dilution; however this approach has a number of drawbacks. Today, there is an alternative solution which eliminates these limitations: the HP D300 Digital Dispenser.
App Note / Case Study
Tag-lite® two-cell assay: a valuable tool for protein drug discovery
Antibodies are central mediators of the immune system, IgG being the most dominant. Binding of IgG/antigen complexes to membrane-bound Fc? receptors triggers a cellular immune response. Alterations in the Fc portion of the antibody by introducing mutations can lead to complete abolition of Fc? receptor binding.
This describes the development of a cellbased TR-FRET assay to determine the simultaneous binding of an antibody to its antigen and huFc?RIIIa present on different cells.
App Note / Case Study
Transcreener® ADP2 TR-FRET Red Assay
Adenosine diphosphate (ADP) is an important mediator of cellular metabolism that is essential for maintaining energy levels.
The Transcreener ADP2 TR-FRET Red Assay is intended for the detection of ADP production by any kinase or ATPase, and has been developed specifically for high throughput screening applications.
App Note / Case Study
3D Cell Culture: mimicking real tissue
Technological advances in the last couple of years have enabled 3D scaffolds to be engineered that offer a real proxy for the in vivo environment which can be easily and routinely used in a cell culture laboratory.
Alvetex Scaffold enables cells to develop natural, in vivo-like intercellular interactions, providing an ideal environment for real three-dimensional growth and nutrient exchange that is comparable to intra-capillary exchange in living tissues.
Alvetex Scaffold enables cells to develop natural, in vivo-like intercellular interactions, providing an ideal environment for real three-dimensional growth and nutrient exchange that is comparable to intra-capillary exchange in living tissues.
App Note / Case Study
Colony Picking - fully automated screening of fungi
Manual screening processes are very costly and time consuming. This ambitious project for high throughput screening (HTS) of cellulase-producing fungal strains demonstrates the high capacity and unique features of the Freedom EVO colony picking workstation. The picking of fungi is more complex than for typical bacterial colonies, and requires advanced selection criteria to be combined with complex pipetting and a tip movement that mimics the manual picking process.
App Note / Case Study
3D cell culture: a realistic and reproducible collagen-based environment
Researchers are increasingly turning to 3D cell culture techniques, due to the improved physiological relevance of the cellular environment. 3D cell cultures offer a more in vivo-like response to stimuli more closely resembling the behavior of animal models or the human body.
App Note / Case Study
Optimized cell culture
Culturing of various stable cell lines, including bacterial, yeast, hybridoma, insect and mammalian lines, and stem cells, is an important task in the pharmaceutical industry. The cells need to be maintained and expanded for use in cell-based assays, such as siRNA screening, ADME and high content screening, as well as for clone selection.
App Note / Case Study
Efficient washing of magnetic beads
The use of magnetic beads as the solid phase in a multiplexed ELISA enables high throughput cytokine screening and offers several advantages over common ELISA assays with direct coupling of the analyte to the well-surface.
App Note / Case Study
Determination of microbial cell viability
The BacTiter-Glo Microbial Cell Viability Assay is a luminescence-based assay for the determination of the viability of microbial cells, using quantification of ATP as an indicator of metabolically active, viable cells. The assay protocol comprises the addition of the BacTiter-Glo reagent directly to the sample, and subsequent measurement of luminescence; cell washing, removal of culture medium, and multiple pipetting steps are not required.
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