Creating dose-response curves for cell-based and biochemical assays with the HP D300 Digital Dispenser
The HP D300 Digital Dispenser has enabled the test compounds to be assessed in two different assays – one cell-based and one biochemical. The results are consistent with an expertly performed manual process, but with the benefit of significant time savings, the elimination of serial dilution, higher quality data, and a reduction in compound usage. Moreover, the improved precision offered by the HP D300 enables easier and more rapid generation of data.
This application note compares the results of dose-response curves set up using the HP D300 Digital Dispenser and a traditional manual serial dilution method in two different assays: the LANCE® cell-based cAMP assay and a biochemical assay for nuclear receptor activation by AlphaScreen®.