Determination of microbial cell viability
Application Note Feb 27, 2014
Tecan Group Ltd.
The chemistry of the assay relies on the properties of a thermostable luciferase (Ultra-Glo™ Recombinant Luciferase) and a proprietary formulation for extracting ATP from bacteria. The luminescent signal generated is proportional to the amount of ATP, and therefore an indicator of the number of viable cells in the sample. The ‘glow-type’ nature of the assay permits signal measurement over a period of approximately 30 minutes, depending on the type of bacteria and growth medium.
The BacTiter-Glo Microbial Cell Viability Assay was tested on the Infinite F200 PRO filter-based multimode reader, using the instrument’s highly sensitive luminescence module.