Fully Automated 24-Well Madin-Darby Canine Kidney (MDCK) Permeability Assay Utilizing the Hamilton Microlab® Star Liquid Handling Platform

Permeability assays are used to elucidate the structure-activity relationships in the hit-to-lead stage, rank order compounds for in vivo pharmacokinetics studies, and predict the potential for oral absorption. It is important to have reliability, accuracy and precision for such assays especially when used in mid-high throughput screening. This application note describes the fully automated 24-well Madin-Darby Canine Kidney (MDCK) permeability assay using the MICROLAB STAR liquid handling workstation.