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Overcoming the Hard-to-Transfect Cell Line Hurdle

Cell Line App note

In the era of CRISPR/Cas9 technology, the ability to generate gene knockouts to understand gene and protein function is revolutionary in biomedicine and drug discovery. However, certain cells are hard to transfect and challenges remain in the singulation and expansion of edited cells for monoclonal lines. 

This app note presents an efficient solution to enable researchers perform gene targeting in cells that are considered hard-to-transfect. 

Download this app note to discover:

  • Optimized onboarding for efficient single-cell transfection
  • Gentle intranuclear delivery for hard-to-transfect cells
  • Step-by-step methodology for knockout cell line generation
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