Reach New Levels of Metabolite Characterization
Early ADME studies are used to determine if a drug candidate is prone to metabolic breakdown or oxidation/conjugation. Glucuronide conjugation is difficult to characterize using MS/MS alone, therefore fragmentation is also required to obtain structural information.
Electron-activated dissociation (EAD) is an orthogonal fragmentation technique that can generate fragments of glucuronide conjugates that retain the glucuronic acid group, allowing for a confident assessment of the conjugation site.
Download this app note to:
- Obtain richer fragmentation patterns
- Generate diagnostic spectral peaks for labile fragments
- Confidently differentiate between conjugation sites