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Size-selective assessment of tight junction paracellular permeability using fluorescently labelled dextrans


A method for the quick and reliable analysis of size-selective paracellular tracer diffusion using fluorescent dextrans of different molecular weights is described. The assay could be easily adapted to large scale screens to identify genome-wide regulatory pathways or small molecules to modify junctional permeability. Using BMG LABTECH´s  multimode plate reader FLUOstar OPTIMA for these experiments allows for quick and consistent fluorescence measurements in either kinetic or endpoint format.