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Targeted Enrichment for Methylation Analysis

gene regulation
Credit: iStock

Methylation sequencing is an important tool for understanding gene regulation and is increasingly used in translational research, including in assays for better understanding neurological and developmental disorders.

However, detecting methylation through next-generation sequencing can be complex, time-intensive and costly. Custom capture panels enable a more cost-effective methylation profiling solution that maximizes both the number of samples analyzed and the depth of sequencing.

This application note reviews a simple and effective workflow used to study differences in methylation status in mice brain and blood cells.  

Download this app note to discover:

  • Insights into best practices for sample preparation and data analysis when using custom panel
  • How to increase throughput while minimizing costs
  • An expert-designed sequencing protocol from sample collection through to data interpretation

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