Using the MVS to Measure Residual Volumes Remaining in a Plate After Sample Aspiration: General Approach

The purpose of this application note is to demonstrate an alternative use for the Artel MVS® Multichannel Verification System. As opposed to measuring the accuracy and precision for a volume transfer of sample into a microtiter plate, the MVS is instead used to measure resididual liquid after sample is aspirated out of each well.

In most scenarios, the MVS is employed to measure the accuracy and precision of a dispensed, or transferred, sample volume within a microplate. This study demonstrates a related, but unique, application: using the MVS to measure the amount of residual volume left behind within a well of a plate after some or all of the sample volume is aspirated out. The experiments discussed herein illustrate the use of the MVS to measure small, residual volumes following one or more removal steps of aqueous sample solutions.