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Top Tips for Consistent qPCR

Sample tubes are placed into a PCR instrument.
Credit: iStock

Quantitative real-time PCR (qPCR) has become a staple of molecular biology laboratories worldwide, with a broad range of applications including gene expression analysis, single nucleotide polymorphism genotyping, pathogen detection, drug discovery and clinical diagnosis.


Careful experimental design with appropriate positive and negative controls is extremely important to obtain reliable results in a qPCR experiment. 


Download this guide to learn more about:
  • Designing qPCR experiments
  • Endogenous, negative and positive controls
  • Data analysis and ensuring good data quality

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