In situ RNA Hybridization for Drug Discovery: 8 Considerations
List Aug 02, 2018 | by Masha G. Savelieff, PhD, SciGency
In situ RNA hybridization has been an important method in the researcher’s toolbox for decades, and it is being continually refined to increase its scope.
The technique utilizes a probe, which has a complementary, anti-sense sequence that is unique to the target RNA transcript it will hybridize with, to detect an RNA transcript of interest within cells, tissue, or organ slices.
This list highlights 8 points to consider when using in situ RNA hybridization for drug discovery.
Biotherapeutics are highly complex molecules, needing detailed analysis to determine how they will behave in the human body. Characterization of the biotherapeutic is needed at each stage of the drug development process and there are a variety of analytical characterization techniques available. This list highlights five approaches.