We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
Determination of Phosphate in Soil Extracts in the Field: A Green Chemistry Enzymatic Method
News

Determination of Phosphate in Soil Extracts in the Field: A Green Chemistry Enzymatic Method

Determination of Phosphate in Soil Extracts in the Field: A Green Chemistry Enzymatic Method
News

Determination of Phosphate in Soil Extracts in the Field: A Green Chemistry Enzymatic Method

Read time:
 

Want a FREE PDF version of This News Story?

Complete the form below and we will email you a PDF version of "Determination of Phosphate in Soil Extracts in the Field: A Green Chemistry Enzymatic Method"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Measurement of ortho-phosphate in soil extracts usually involves sending dried samples of soil to a laboratory for analysis and waiting several weeks for the results. Phosphate determination methods often involve use of strong acids, heavy metals, and organic dyes. To overcome limitations of this approach, we have developed a phosphate determination method which can be carried out in the field to obtain results on the spot.

This new method uses:

• Small volumes.

• An enzymatic reaction.

• Green chemistry.

First, the soil sample is extracted with deionized water and filtered. Next, an aliquot of the soil extract (0.5 mL) is transferred to a disposable cuvette, containing 0.5 mL of reaction mixture [200 mM HEPES, pH 7.6, 20 mM MgCl2, with 80 nmol 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) and 1 unit of recombinant purine nucleoside phosphorylase (PNP; EC 2.4.2.1)], mixed, and incubated for 10 min at field temperature. Absorbance of the completed reaction is measured at 360 nm in open-source, portable photometer linked by bluetooth to a smartphone. The phosphate and phosphorus content of the soil is determined by comparison of its absorbance at 360 nm to a previously prepared standard phosphate curve, which is stored in the smartphone app.

Advertisement