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Automated identification of lipids using CRF ions and high energy MS/MS fragment ions and novel software.

Automated identification of lipids using CRF ions and high energy MS/MS fragment ions and novel software.

Purpose: Assess the feasibility of using analyte protectants to improve the analysis of benzodiazepines.

Purpose: Facilitate intact protein LC-MS without compromising performance.
Methods: Construct prototypes of integrated column and sprayer dedicated for intact protein separations and compare performance.
Results: Three prototypes have been directly compared and all showed good protein separations, each with their unique area of strength.

The best of CBF/imgCIF (the Crystallographic Binary File, which has a complete description of the experiment), NeXus (a common data framework for neutron, X-ray and muon science, which gracefully handles large data sets) and HDF5 (Hierarchical Data Format, version 5, the high-performance data format used by NeXus) are being combined for the management of BIG DATA at synchrotrons.

miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.

High-throughput analysis of protein formulations by Dynamic Light Scattering (DLS): thermal stability, colloidal stability and a thermal anomaly in the colloidal stability parameter D₁

Sagittal, coronal and horizontal, one micron thick plastic (semithin) sections of adult zebrafish (Danio rerio) brain were photographed in 40X. The sections were then photomontaged together using in Adobe Photoshop CS5 Extended Version 12.0 to make the photographic atlas.

Many types of chemical and biological analyses are based upon analytical techniques involving accurate delivery of liquid components. Pipetting tools have become commonplace especially in many biological and pharmaceutical laboratories. While these tools are familiar to many, their operational differences when pipetting different types of solutions are often over-looked.

A successful microwave synthesis of methyl salicylate to aspirin using green catalysts from over the counter pain creams. The process was also prepared and tested in an undergraduate organic chemistry lab.