Carbon Nanotubes by Microwave Plasma Enhanced Chemical Vapor Deposition
MICROWAVE Plasma generates radical density characteristic of 2000C at much lower effective temperature and can be highly ionized (Low pressure ECR mode) or highly neutral (high pressure mode).
Miniaturization of Protein Kinase Assays in the MultiScreen®HTS 384-Well P81 Phosphocellulose Filter Plate
In this study, using Protein Kinase A (PKA) as are presentative assay system, peptide phosphorylation reactions carried out in 384 PH filter plates showed dependence on enzyme and substrate concentrations, and on time. Kinase inhibition studies demonstrated IC50 and Z’ values equivalent to those obtained in 96-well plates. The higher throughput 384 well format offers a miniaturized version of the 96 well assay with equivalent sensitivity and reproducibility.
Metabonomics for MolPAGE Discovering Diabetes Biomarkers
MolPAGE (Molecular Phenotyping to Accelerate Genomic Epidemiology) is an EU consortium with almost twenty collaborating universities and companies throughout Europe. One of the aims of MolPAGE is to find early onset biomarkersfor type 2 diabetes (T2DM) and cardio-vascular diseases(CVD).
Development of low Cost High Throughput miRNA Screening
Micro-RNAs (miRNAs) form a class of 17-24 base singles trandnon-coding RNAs which regulate gene expression post-transcriptionally in eukaryotes and viruses. We have developed a low cost high throughput method to quantify the expression of these miRNAs via classictotal RNA extraction without interference from genomic DNA.
A Mild Protocol for Parallel Solution Phase Synthesis of Cyclic Imides
The combination of using high temperatures and coupling reagents allows production of cyclic imides rapidly and in higher purity than heat or coupling agents alone.
Microwave-Accelerated Fischer Glycosidation
Fischer glycosidation appeared an ideal candidate for the application of microwave irradiation and herein we report a systematic analysis of the effect of microwave heating in the Fischer glycosidation reaction.
High-Throughput Automation of a Dual Reporter Assay in Low Volume 384 & 1536 Well Plate Formats
The drive towards miniaturization within the pharmaceutical and biotechnology fields has created a need for liquid handling technologies that accurately deliver low volume reagents to high-density plates. This has also created a need for simple, fully scaleable assays in low volumes. Here we demonstrate the successful combination of both through the use of the Equator™ NS 808 - Eight Tip Pipetting System and the dual color Chroma-Luc™ technology.
Functional Characterization of the Human Fractalkine Receptor CX3CR1 Using Photina™, a new Highly Sensitive Photoprotein
CX3CR1 has been stably transfected into CHOmitoPhotina™- MRE-CRE-Luciferase cell line and assays suitable for HTS and uHTS have been developed.
GPVI-RBL-2H3-NFAT-Re Luciferase Cell Line: a New Cell Based System for Studying Collagen Receptor Activation
This study describes the generation of a cell based assay which allows the functional characterization of the human GPVI receptor, by the use ofluminescence.
High Density Receptor Ligand Binding Assays in the MultiScreen®HTS 384-well Glass Fiber Filter Plate
Using the Muscarinic M1 G-protein Coupled Receptor as a model system, we achieved accurate and reproducible determinations of binding affinity (Kd) and IC50 results for known ligands on the 384-well plate device. As a result of being able to incubate the reaction mixture in the filter plate and configure the assay using half the reaction volume, significant reductions in reagent costs and radioactive waste were achieved.